Mode of activation of the GC box/Spl-dependent promoter of the human NADH-cytochrome b5 reductase-encoding gene

Atsushi Toyoda*, Yasuyuki Fukumaki, Masahira Hattori, Yoshiyuki Sakaki

*この研究の対応する著者

研究成果: Article査読

15 被引用数 (Scopus)

抄録

Many eukaryote promoters, particularly those for so-called housekeeping genes, have multiple GC boxes which are the binding sites of the transcription factor, Spl. It has been proposed that Spl binds to the multiple GC boxes, and then the GC box-bound Spl interact with each other to synergistically stimulate transcription. Here, we describe a Spl-dependent promoter which does not necessarily fit the synergistic activation mechanism. The promoter of the human NADH-cytochrome b5 reductase-encoding gene (CYTB5R) possesses five potential GC box sequences. Deletion and mutagenesis studies coupled with CAT assays revealed that three out of five GC box-like sequences were functionally active and activated transcription additively (rather than synergistically). Our results suggested that Spl-mediated activation of transcription occurs in a promoter context-dependent manner.

本文言語English
ページ(範囲)351-355
ページ数5
ジャーナルGene
164
2
DOI
出版ステータスPublished - 1995 10 27
外部発表はい

ASJC Scopus subject areas

  • 遺伝学

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