Modulation of eosinophil chemotactic activities to leukotriene B4 by n-3 polyunsaturated fatty acids

S. Kikuchi, T. Sakamoto, C. Ishikawa, Kazunaga Yazawa, S. Torii

研究成果: Article

9 引用 (Scopus)

抜粋

Eosinophil accumulation induced by leukotriene B4 appears to be involved in the pathogenesis of allergic diseases. We evaluated the effects of docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) on chemotaxis to leukotriene B4 in guinea pig peritoneal eosinophils. Guinea pigs that were sensitized to polymyxin B were administered an intraperitoneal injection of polymyxin B (1 mg/animal) alone or combined with DHA (15 or 50 mg/kg, i.p.), EPA (50 or 100 mg/kg, i.p.), or with linoleic acid (LA) (100 mg/kg, i.p.). Forty hours later, eosinophils were obtained from the intraperitoneal ravage fluid and purified. The chemotactic and chemokinetic responses of eosinophils to leukotriene 3, were measured using a 96-well microchemotaxis chamber. DHA significantly decreased the chemotactic and chemokinetic responses of eosinophils in a dose-dependent fashion. A higher dose of EPA also significantly inhibited both of those responses, whereas LA had no effect. Our results suggested a possible mechanism for the improvement of allergic diseases by dietary supplementation with n-3 PUFA.

元の言語English
ページ(範囲)243-248
ページ数6
ジャーナルProstaglandins Leukotrienes and Essential Fatty Acids
58
発行部数3
DOI
出版物ステータスPublished - 1998 3
外部発表Yes

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Endocrinology, Diabetes and Metabolism

フィンガープリント Modulation of eosinophil chemotactic activities to leukotriene B<sub>4</sub> by n-3 polyunsaturated fatty acids' の研究トピックを掘り下げます。これらはともに一意のフィンガープリントを構成します。

  • これを引用