Modulation of protein-ligand interactions by photocleavage of a cyclic peptide using phosphatidylinositol 3-kinase SH3 domain as model system

Isao Takahashi, Shigeki Kuroiwa, Hanna E. Lindfors, Lionel A. Ndamba, Yoshitaka Hiruma, Tatsuo Yajima, Nobuyuki Okishio, Marcellus Ubbink, Shun Hirota*

*この研究の対応する著者

研究成果: Article査読

5 被引用数 (Scopus)

抄録

To photomodulate the interaction of the phosphatidylinositol 3-kinase SH3 domain with a peptide ligand, a cyclic peptide (cyclic-1)with a photolabile side chain-to-side chain linkerwas synthesized. The conformation of cyclic-1 differs from that of the parent linear peptide, but becomes identical by UV-irradiation. Accordingly, the binding affinity of cyclic-1 to the SH3 domain increased upon conversion of the cyclic to a linear flexible structure by irradiation (Kd: 3.4 ± 1.7 and 0.9 ± 0.3mM, respectively). These results confirm the usefulness of a photocleavable peptide for photocontrol of peptide-protein interactions.

本文言語English
ページ(範囲)411-416
ページ数6
ジャーナルJournal of Peptide Science
15
6
DOI
出版ステータスPublished - 2009
外部発表はい

ASJC Scopus subject areas

  • 構造生物学
  • 生化学
  • 分子医療
  • 分子生物学
  • 薬理学
  • 創薬
  • 有機化学

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