Molecular cloning and chromosomal localization of the human thrombopoietin gene

Yoshiaki Sohma, Hiromichi Akahori, Naohiko Seki, Tada aki Hori, Kinya Ogami, Takashi Kato, Yoshihiro Shimada, Kazuo Kawamura*, Hiroshi Miyazaki

*この研究の対応する著者

研究成果: Article査読

238 被引用数 (Scopus)

抄録

The complete gene for human thrombopoietin (TPO) has been cloned by screening a human genomic library using human TPO cDNA as a probe. This gene is 6.2 kb in length and contains six exons and five introns. It is shown that the human genome contains a single copy of the human TPO gene according to Southern blotting analysis. The transcription initiation site was determined by S1 nuclease mapping. The human TPO gene expressed TPO activity when transfected into COS-1 cells. The human TPO gene has been mapped to chromosome 3q27 by in situ hybridization using a biotin-labeled probe.

本文言語English
ページ(範囲)57-61
ページ数5
ジャーナルFEBS Letters
353
1
DOI
出版ステータスPublished - 1994 10 10
外部発表はい

ASJC Scopus subject areas

  • 生物理学
  • 構造生物学
  • 生化学
  • 分子生物学
  • 遺伝学
  • 細胞生物学

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