TY - JOUR
T1 - Molecular cloning and chromosomal mapping of the mouse gene encoding cyclin-dependent kinase 5 regulatory subunit p35
AU - Ohshima, Toshio
AU - Kozak, Christine A.
AU - Nagle, James W.
AU - Pant, Harish C.
AU - Brady, Roscoe O.
AU - Kulkarni, Ashok B.
N1 - Funding Information:
*Developmental and Metabolic Neurology Branch, §Neurogenetic Section, and ØLaboratory of Neurochemistry, National Institute of Neurological Disorders and Stroke; ²Gene Targeting Research and Core Facility, National Institute of Dental Research; and ³Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892-4326
PY - 1996/7/15
Y1 - 1996/7/15
N2 - A neural-specific activating subunit, p35, of cyclin-dependent kinase 5 (Cdk5) was recently reported to differ from other mammalian cyclins, suggesting a new type of regulatory subunit for Cdk activity. The mouse gene encoding p35, Cdk5r, was isolated from a mouse 129/SvJ genomic library, and the genomic structure of Cdk5r was characterized. The most notable features of Cdk5r are the absence of introns in the amino acid coding region and the high homology of amino acid sequence among species. The 5'-flanking region of Cdk5r contained no canonical TATA or CAAT box but had several putative promoter elements, including Sp1, AP2, MRE, and NGFIA. The mouse Cdk5r transcript was detected only in the brain by Northern blot analysis. Mouse Cdk5r was mapped to a position on mouse chromosome 11.
AB - A neural-specific activating subunit, p35, of cyclin-dependent kinase 5 (Cdk5) was recently reported to differ from other mammalian cyclins, suggesting a new type of regulatory subunit for Cdk activity. The mouse gene encoding p35, Cdk5r, was isolated from a mouse 129/SvJ genomic library, and the genomic structure of Cdk5r was characterized. The most notable features of Cdk5r are the absence of introns in the amino acid coding region and the high homology of amino acid sequence among species. The 5'-flanking region of Cdk5r contained no canonical TATA or CAAT box but had several putative promoter elements, including Sp1, AP2, MRE, and NGFIA. The mouse Cdk5r transcript was detected only in the brain by Northern blot analysis. Mouse Cdk5r was mapped to a position on mouse chromosome 11.
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U2 - 10.1006/geno.1996.0370
DO - 10.1006/geno.1996.0370
M3 - Article
C2 - 8661152
AN - SCOPUS:0030586241
SN - 0888-7543
VL - 35
SP - 372
EP - 375
JO - Genomics
JF - Genomics
IS - 2
ER -