Molecular cloning and expression of long-wavelength-sensitive cone opsin in the brain of a tropical damselfish

Yuki Takeuchi, Mohammad Abu Jafor Bapary, Syugo Igarashi, Satoshi Imamura, Yuji Sawada, Mio Matsumoto, Sung Pyo Hur, Akihiro Takemura

研究成果: Article

10 引用 (Scopus)

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Ovarian development of the sapphire devil, Chrysiptera cyanea, exhibits photoperiodism and is stimulated under long-day conditions. Previous studies suggest that red light is more effective than green and blue lights for inducing ovarian development. In addition, the extra-retinal photoreception involved in the ovarian development is suggested in this species. The present study aimed to clone the red-light-sensitive cone opsin (sdLWS) of this species, to demonstrate its expression in the brain, and to confirm the effectiveness of red light on the initiation of ovarian development. A phylogenetic analysis revealed that sdLWS belongs to the long-wavelength-sensitive opsin (LWS opsin) group, showing high identity (77-92%) with the LWS opsin of other fishes. RT-PCR showed that sdLWS is expressed in the eye, brain, and ovary. In situ hybridization indicated that sdLWS is expressed in the third ventricle periventricular area in the anterior hypothalamus. Exposing fish to long-day conditions of red light resulted in the appearance of vitellogenic oocytes in the ovary and an increase in the gonadosomatic index within 2. weeks, suggesting that fish immediately undergo oocyte development under red light conditions. These results indicate that sdLWS is a possible candidate of deep brain photoreceptor molecule involved in photoperiodic ovarian development in the sapphire devil.

元の言語English
ページ(範囲)486-492
ページ数7
ジャーナルComparative Biochemistry and Physiology - A Molecular and Integrative Physiology
160
発行部数4
DOI
出版物ステータスPublished - 2011 12
外部発表Yes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Physiology

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