抄録
A cDNA clone encoding preprorelaxin was isolated from the equine placental cDNA library and its nucleotide sequence was determined. Analysis of the sequence revealed that the cDNA contained a polyadenylation site and an open reading frame encoding 182 amino acids, including a signal peptide of 25 amino acids, a B-chain of 28, a C-peptide of 109 and an A-chain of 20 amino acids. The nucleotide sequence of preprorelaxin shows homology with those of the pig (75.5%), monkey (67.4%), human (67.4%), rat (60.7%) and mouse (54.6%). The main differences between equine preprorelaxin and that of these other species were the insertion of a Leu residue at position 87 in the C-peptide and also the deletion of two residues from the C-peptide and A-chain. Northern blot analysis of mRNA obtained from pregnancy day 70 placenta showed that there were two different sizes of mRNA (1.0 and 1.8 kb).
本文言語 | English |
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ページ(範囲) | 171-178 |
ページ数 | 8 |
ジャーナル | Journal of Reproduction and Development |
巻 | 42 |
号 | 3 |
出版ステータス | Published - 1996 |
外部発表 | はい |
ASJC Scopus subject areas
- 動物科学および動物学