Molecular cloning of equine preprorelaxin cDNA

Kwan Sik Min, Kunio Shiota*, Tomoya Ogawa

*この研究の対応する著者

研究成果: Article査読

3 被引用数 (Scopus)

抄録

A cDNA clone encoding preprorelaxin was isolated from the equine placental cDNA library and its nucleotide sequence was determined. Analysis of the sequence revealed that the cDNA contained a polyadenylation site and an open reading frame encoding 182 amino acids, including a signal peptide of 25 amino acids, a B-chain of 28, a C-peptide of 109 and an A-chain of 20 amino acids. The nucleotide sequence of preprorelaxin shows homology with those of the pig (75.5%), monkey (67.4%), human (67.4%), rat (60.7%) and mouse (54.6%). The main differences between equine preprorelaxin and that of these other species were the insertion of a Leu residue at position 87 in the C-peptide and also the deletion of two residues from the C-peptide and A-chain. Northern blot analysis of mRNA obtained from pregnancy day 70 placenta showed that there were two different sizes of mRNA (1.0 and 1.8 kb).

本文言語English
ページ(範囲)171-178
ページ数8
ジャーナルJournal of Reproduction and Development
42
3
出版ステータスPublished - 1996
外部発表はい

ASJC Scopus subject areas

  • 動物科学および動物学

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