Monitoring the microbial population dynamics at the start-up stage of wastewater treatment reactor by terminal restriction fragment length polymorphism analysis based on 16s rDNA and rRNA gene sequences

Takeshi Terahara, Tatsuhiko Hoshino, Satoshi Tsuneda, Akira Hirata, Yuhei Inamori

研究成果: Article

17 引用 (Scopus)

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The microbial population dynamics at the start-up stage of a wastewater treatment reactor was investigated using terminal restriction fragment length polymorphism (T-RFLP) analysis based on 16S rDNA and rRNA gene sequences. The results of fragment peaks suggested that the number and activity of nitrifying bacteria increased in association with the start of nitrification, and the relative ratios of 16S rRNA of these bacteria changed prior to those of the 16S rDNA. Furthermore, multidimensional scaling (MDS) analysis revealed that the 16S rRNA exhibited wider dispersion than the 16S rDNA at the start-up stage, indicating that the diversity of 16S rRNA in the microbial communities was strongly affected by environmental changes.

元の言語English
ページ(範囲)425-428
ページ数4
ジャーナルJournal of Bioscience and Bioengineering
98
発行部数6
DOI
出版物ステータスPublished - 2004 12

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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