Monitoring the microbial population dynamics at the start-up stage of wastewater treatment reactor by terminal restriction fragment length polymorphism analysis based on 16s rDNA and rRNA gene sequences

Takeshi Terahara, Tatsuhiko Hoshino, Satoshi Tsuneda, Akira Hirata, Yuhei Inamori

    研究成果: Article

    17 引用 (Scopus)

    抄録

    The microbial population dynamics at the start-up stage of a wastewater treatment reactor was investigated using terminal restriction fragment length polymorphism (T-RFLP) analysis based on 16S rDNA and rRNA gene sequences. The results of fragment peaks suggested that the number and activity of nitrifying bacteria increased in association with the start of nitrification, and the relative ratios of 16S rRNA of these bacteria changed prior to those of the 16S rDNA. Furthermore, multidimensional scaling (MDS) analysis revealed that the 16S rRNA exhibited wider dispersion than the 16S rDNA at the start-up stage, indicating that the diversity of 16S rRNA in the microbial communities was strongly affected by environmental changes.

    元の言語English
    ページ(範囲)425-428
    ページ数4
    ジャーナルJournal of Bioscience and Bioengineering
    98
    発行部数6
    出版物ステータスPublished - 2004 12

    Fingerprint

    Population dynamics
    Population Dynamics
    Waste Water
    Ribosomal DNA
    Polymorphism
    rRNA Genes
    Restriction Fragment Length Polymorphisms
    Wastewater treatment
    Bacteria
    Genes
    Nitrification
    Monitoring
    Association reactions

    ASJC Scopus subject areas

    • Biotechnology
    • Bioengineering

    これを引用

    @article{229f70b601ef4041816d567ffff0863f,
    title = "Monitoring the microbial population dynamics at the start-up stage of wastewater treatment reactor by terminal restriction fragment length polymorphism analysis based on 16s rDNA and rRNA gene sequences",
    abstract = "The microbial population dynamics at the start-up stage of a wastewater treatment reactor was investigated using terminal restriction fragment length polymorphism (T-RFLP) analysis based on 16S rDNA and rRNA gene sequences. The results of fragment peaks suggested that the number and activity of nitrifying bacteria increased in association with the start of nitrification, and the relative ratios of 16S rRNA of these bacteria changed prior to those of the 16S rDNA. Furthermore, multidimensional scaling (MDS) analysis revealed that the 16S rRNA exhibited wider dispersion than the 16S rDNA at the start-up stage, indicating that the diversity of 16S rRNA in the microbial communities was strongly affected by environmental changes.",
    keywords = "Microbial population dynamics, Multidimensional scaling (MDS), Start-up stage, Terminal restriction fragment length polymorphism (T-RFLP), Wastewater treatment reactor",
    author = "Takeshi Terahara and Tatsuhiko Hoshino and Satoshi Tsuneda and Akira Hirata and Yuhei Inamori",
    year = "2004",
    month = "12",
    language = "English",
    volume = "98",
    pages = "425--428",
    journal = "Journal of Bioscience and Bioengineering",
    issn = "1389-1723",
    publisher = "Elsevier",
    number = "6",

    }

    TY - JOUR

    T1 - Monitoring the microbial population dynamics at the start-up stage of wastewater treatment reactor by terminal restriction fragment length polymorphism analysis based on 16s rDNA and rRNA gene sequences

    AU - Terahara, Takeshi

    AU - Hoshino, Tatsuhiko

    AU - Tsuneda, Satoshi

    AU - Hirata, Akira

    AU - Inamori, Yuhei

    PY - 2004/12

    Y1 - 2004/12

    N2 - The microbial population dynamics at the start-up stage of a wastewater treatment reactor was investigated using terminal restriction fragment length polymorphism (T-RFLP) analysis based on 16S rDNA and rRNA gene sequences. The results of fragment peaks suggested that the number and activity of nitrifying bacteria increased in association with the start of nitrification, and the relative ratios of 16S rRNA of these bacteria changed prior to those of the 16S rDNA. Furthermore, multidimensional scaling (MDS) analysis revealed that the 16S rRNA exhibited wider dispersion than the 16S rDNA at the start-up stage, indicating that the diversity of 16S rRNA in the microbial communities was strongly affected by environmental changes.

    AB - The microbial population dynamics at the start-up stage of a wastewater treatment reactor was investigated using terminal restriction fragment length polymorphism (T-RFLP) analysis based on 16S rDNA and rRNA gene sequences. The results of fragment peaks suggested that the number and activity of nitrifying bacteria increased in association with the start of nitrification, and the relative ratios of 16S rRNA of these bacteria changed prior to those of the 16S rDNA. Furthermore, multidimensional scaling (MDS) analysis revealed that the 16S rRNA exhibited wider dispersion than the 16S rDNA at the start-up stage, indicating that the diversity of 16S rRNA in the microbial communities was strongly affected by environmental changes.

    KW - Microbial population dynamics

    KW - Multidimensional scaling (MDS)

    KW - Start-up stage

    KW - Terminal restriction fragment length polymorphism (T-RFLP)

    KW - Wastewater treatment reactor

    UR - http://www.scopus.com/inward/record.url?scp=12444293836&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=12444293836&partnerID=8YFLogxK

    M3 - Article

    C2 - 16233731

    AN - SCOPUS:12444293836

    VL - 98

    SP - 425

    EP - 428

    JO - Journal of Bioscience and Bioengineering

    JF - Journal of Bioscience and Bioengineering

    SN - 1389-1723

    IS - 6

    ER -