Monoclonal antibody analysis of phosphatidylserine and protein kinase C localizations in developing rat cerebellum

Atsuo Miyazawa, Hiroko Inoue, Tohru Yoshioka*, Tetsuro Horikoshi, Keiji Yanagisawa, Masato Umeda, Keizo Inoue

*この研究の対応する著者

    研究成果: Article査読

    12 被引用数 (Scopus)

    抄録

    Understanding the topographical relationships between phosphatidylserine (PS) and protein kinase C (PKC) within neurons can provide clues about the mechanism of translocation and activation of PKC. For this purpose we applied monoclonal antibodies (Abs) of PS and PKC to sections of developing rat cerebellum. The anti-PKC Ab immunohistochemical pattern showed homogeneous staining of Purkinje cells over various postnatal ages, whereas the anti-PS Ab staining showed a heterogeneous localization over these ages. Purkinje cells did not stain well between postnatal day 14 (PND 14) and PND 21, suggesting that the PS was lost from the membrane during preparation of the sections during this period. These data imply that interactions between PS and PKC vary in Purkinje cells during postnatal development.

    本文言語English
    ページ(範囲)1547-1554
    ページ数8
    ジャーナルJournal of Neurochemistry
    59
    4
    出版ステータスPublished - 1992 10月

    ASJC Scopus subject areas

    • 生化学
    • 細胞および分子神経科学

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