Multiple amino acid-excluded genetic codes for protein engineering using multiple sets of tRNA variants

Kazuaki Amikura, Yoko Sakai, Shun Asami, Daisuke Kiga*

*この研究の対応する著者

研究成果: Article査読

6 被引用数 (Scopus)

抄録

A "simplified genetic code", with only 19 amino acids assigned to the sense codons, was recently developed. In this study, we describe novel simplified codes in which multiple amino acids are simultaneously excluded from the universal code. In the simplest code, tryptophan, cysteine, tyrosine, and asparagine codons are assigned to serine by using four kinds of tRNA Ser variants. The results revealed that various sets of amino acids can easily be excluded from the universal code, using our strategy for genetic code simplification. A simplified genetic code is useful as an engineering tool for the improvement of industrial enzymes and pharmaceuticals, and also provides new insights into the assessment of protein evolution. Simplified codes in which multiple amino acids are simultaneously excluded from the code can be more effective tools than codes excluding only one amino acid.

本文言語English
ページ(範囲)140-144
ページ数5
ジャーナルACS Synthetic Biology
3
3
DOI
出版ステータスPublished - 2014 3月 21
外部発表はい

ASJC Scopus subject areas

  • 生体医工学
  • 生化学、遺伝学、分子生物学(その他)

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