Multiple myosin II heavy chain kinases: Roles in filament assembly control and proper cytokinesis in Dictyostelium

Shigehiko Yumura, Masashi Yoshida, Venkaiah Betapudi, Lucila S. Licate, Yoshiaki Iwadate, Akira Nagasaki, Taro Q.P. Uyeda, Thomas T. Egelhoff*

*この研究の対応する著者

研究成果: Article査読

70 被引用数 (Scopus)

抄録

Myosin II filament assembly in Dictyostelium discoideum is regulated via phosphorylation of residues located in the carboxyl-terminal portion of the myosin II heavy chain (MHC) tail. A series of novel protein kinases in this system are capable of phosphorylating these residues in vitro, driving filament disassembly. Previous studies have demonstrated that at least three of these kinases (MHCK A, MHCK B, and MHCK C) display differential localization patterns in living cells. We have created a collection of single, double, and triple gene knockout cell lines for this family of kinases. Analysis of these lines reveals that three MHC kinases appear to represent the majority of cellular activity capable of driving myosin II filament disassembly, and reveals that cytokinesis defects increase with the number of kinases disrupted. Using biochemical fractionation of cytoskeletons and in vivo measurements via fluorescence recovery after photobleaching (FRAP), we find that myosin II overassembly increases incrementally in the mutants, with the MHCK A-/B -/C- triple mutant showing severe myosin II overassembly. These studies suggest that the full complement of MHC kinases that significantly contribute to growth phase and cytokinesis myosin II disassembly in this organism has now been identified.

本文言語English
ページ(範囲)4256-4266
ページ数11
ジャーナルMolecular biology of the cell
16
9
DOI
出版ステータスPublished - 2005 9月
外部発表はい

ASJC Scopus subject areas

  • 分子生物学
  • 細胞生物学

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