Multiple reporter gene assays for the assessment and estimation of chemical toxicity.

Junko Takahashi*, Hitoshi Iwahashi

*この研究の対応する著者

研究成果: Article査読

1 被引用数 (Scopus)

抄録

To detect chemical toxicity, we are making new bioassay systems that use promoters selected from yeast DNA microarray experiments. We performed multiple reporter gene assays using the promoters of these genes; the promoter regions were inserted upstream of green fluorescence protein (GFP). In this report, six genes (HSP26, MET17, YLL057C, FIT2, CUP1 and OYE3) were selected and assays were carried out for 55 chemicals. The promoters of these genes showed different responses to chemicals within 4 h. This result indicates that this technique enables us to predict the toxicity of chemicals in the environment and to understand toxicities of newly synthesized chemicals.

本文言語English
ページ(範囲)269-282
ページ数14
ジャーナルEnvironmental sciences : an international journal of environmental physiology and toxicology
11
5
出版ステータスPublished - 2004
外部発表はい

ASJC Scopus subject areas

  • 医学(全般)

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