Multiple-site optical recording for characterization of functional synaptic organization of the optic tectum of rainbow trout

Masae Kinoshita, Risa Ueda, Satoshi Kojima, Katsushige Sato, Masahiko Watanabe, Akihisa Urano, Etsuro Ito*

*この研究の対応する著者

研究成果: Article査読

14 被引用数 (Scopus)

抄録

To map the functional synaptic organization over a wide area in the optic tectum, we directly monitored two-dimensional propagation of postsynaptic depolarization evoked by firing of retinotectal afferents in optic tectum slices prepared from rainbow trout (Oncorhynchus mykiss), using a voltage-sensitive dye and a photodiode array system. The postsynaptic responses to afferent stimulation first propagated in the stratum opticum and stratum fibrosum et griseum superficiale in an anterograde fashion in the afferents and then expanded vertically into the deep layers. This vertical propagation appeared to occur along a bundle-like structure that corresponded well with a cluster of neurons whose somata are located in the stratum periventriculare. Pharmacological studies showed that these postsynaptic responses were mediated by ionotropic glutamate receptors. On the other hand, the optical signals appeared to consist of at least two components (a transient signal and a slow signal). The second transient signal summated with the first slow signal by paired stimulation, suggesting that the transient and slow signals originated from different cell types. Taken together, these results showed that the functional synaptic organization of the teleost optic tectum comprises of two depolarization-signal propagating paths along a horizontal layer structure and a vertical bundle-like structure and that these synaptic responses occur via glutamatergic transmission.

本文言語English
ページ(範囲)868-876
ページ数9
ジャーナルEuropean Journal of Neuroscience
16
5
DOI
出版ステータスPublished - 2002 12月 28
外部発表はい

ASJC Scopus subject areas

  • 神経科学(全般)

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