Musashi1, an evolutionarily conserved neural RNA-binding protein, is a versatile marker of human glioma cells in determining their cellular origin, malignancy, and proliferative activity

Y. Kanemura, M. Yamasaki, Y. Kanemura, K. Mori, H. Fujikawa, H. Hayashi, A. Nakano, T. Matsumoto, K. Tamura, N. Arita*, S. I. Sakakibara, T. Ohnishi, S. Fushiki, Y. Nakamura, T. Imai, H. Okano

*この研究の対応する著者

研究成果: Article査読

110 被引用数 (Scopus)

抄録

Tumor cells often express phenotypic markers that are specific to the cells from which they originated. A neural RNA-binding protein, Musashi1, is an evolutionarily well-conserved marker for neural stem cells/progenitor cells. To examine the origin of gliomas, we examined the expression of the human Musashi1 homolog, MSI1, in human glioma tissues and in normal human adult and fetal brains. As we had seen previously in rodents, in the normal human brain, MSI1 was expressed in cells located in the ventricular and subventricular zones, in GFAP-negative glial cells, and in GFAP-positive astrocytes. In glioblastomas, MSI1 was expressed in GFAP-negative tumor cells forming foci that were clearly demarcated and surrounded by GFAP-positive cells. Tumor cells arranged in pseudopalisades were also strongly immunoreactive with MSI1 antibodies. The percentage of MSI1-labeled tumor cells increased in higher-grade astrocytomas and correlated with proliferative activity, as estimated by an MIB-1 staining index. Our results indicate that MSI1 is an excellent marker for neural progenitor cells including neural stem cells in normal human brains. Furthermore, the expression of MSI1 correlates well with the immature nature as well as the malignancy of tumor cells in human gliomas. Thus, we expect the analysis of MSI1 expression to contribute to the understanding of the cellular origin and biology of human gliomas.

本文言語English
ページ(範囲)141-152
ページ数12
ジャーナルDifferentiation
68
2-3
DOI
出版ステータスPublished - 2001
外部発表はい

ASJC Scopus subject areas

  • 分子生物学
  • 発生生物学
  • 細胞生物学
  • 癌研究

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