We prepared series of P2-P4 domain variants of Escherichia coli ribonuclease P (RNase P) ribozyme, and examined enzymatic activities of them. The results indicated that mutations in these domains did not affect the cleavage site selection of the enzyme, but affected cleavage efficiencies and dependence on magnesium ion concentrations.
|ジャーナル||Nucleic acids symposium series (2004)|
|出版ステータス||Published - 2004|
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