Mutational analysis of the length of the J3/4 domain of Escherichia coli ribonuclease P ribozyme

Shinnosuke Haga, Terumichi Tanaka, Yo Kikuchi

研究成果: Article査読

4 被引用数 (Scopus)

抄録

We prepared a series of length variants of the J3/4 domain of Escherichia coli ribonuclease P (RNase P) ribozyme: the four-base long J3/4 domain (A 62G63G64A65) was replaced with GGA (denoted ΔA), GA (ΔAG), A (ΔAGG), AAGGA (ΣA), AAAGGA (ΣAA), and AAAAGGA (ΣAAA). The results indicated that truncating and inserting operations of the J3/4 domain drastically reduced ribozyme activity (WT≫ ΣAA>ΣA>ΣAAA≫ΔAG>ΔA, ΔAGG), but did not affect the cleavage site selection of a substrate by the ribozyme. The reduced ribozyme activity of each mutant was rescued to some extent by the addition of a high concentration of magnesium ions. Our data indicate that the conserved AGGA sequence was important for efficient ribozyme reactions, and suggested that the length mutations affected ribozyme activity through metal ion binding steps.

本文言語English
ページ(範囲)2630-2632
ページ数3
ジャーナルBioscience, Biotechnology and Biochemistry
68
12
DOI
出版ステータスPublished - 2004 12
外部発表はい

ASJC Scopus subject areas

  • Bioengineering
  • Biotechnology
  • Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemistry (miscellaneous)
  • Applied Microbiology and Biotechnology
  • Food Science

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