We have prepared a temporal engineering template for Escherichia coli RNase P RNA that has additional restriction sites on the S-domain region. We have prepared several deletion variants of S-domain region. The results showed the P12 and P13 domains are necessary for efficient enzymatic reactions and the absence of P14 and P17 domains are easily rescued by the presence of high concentration of magnesium ions.
|ジャーナル||Nucleic acids symposium series (2004)|
|出版ステータス||Published - 2007|
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