Nanoscale patterning of protein using electron beam lithography of organosilane self-assembled monolayers

Guo Jun Zhang*, Takashi Tanii, Tamotsu Zako, Takumi Hosaka, Takeo Miyuke, Yuzo Kanari, Takashi Funatsu, Iwao Ohdomari

*この研究の対応する著者

研究成果: Article査読

81 被引用数 (Scopus)

抄録

Nanopatterning of self-assembled monolayers (SAM) by electron beam lithography (EB) was developed to produce organic templates for various nanofabrication processes. EB lithography of SAMs provide a method of patterning substrates with proteins with ultrahigh resolution down to sub-10 nm on the scale of single molecule. Green fluorescent proteins (GFP), which is a spontaneous fluorescence protein isolated from the Pacific Jellyfish was chosen as a model protein. A cysteine residue at the c-terminal end of the protein by site-directed mutagenesis for the site specific modification of protein with biotin was introduced. Field-emission scanning electron microscopy was employed to observe nanostructures composed of two different types of organosilane layers. EB lithography of fluorinated SAM as an ideal template enables the high-resolution patterning of proteins with a high signal-to-noise ratio and integration of bioactive proteins into biosensors.

本文言語English
ページ(範囲)833-837
ページ数5
ジャーナルSmall
1
8-9
DOI
出版ステータスPublished - 2005 8月

ASJC Scopus subject areas

  • バイオテクノロジー
  • 生体材料
  • 化学 (全般)
  • 材料科学(全般)

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