Nanosecond time-resolved fluorescence investigations of temperature-induced conformational changes in cytochrome oxidase in phosphatidylcholine vesicles and solubilized systems

Suguru Kawato*, Satoshi Yoshida, Yutaka Orii, Akira Ikegami, Kazuhiko Kinosita

*この研究の対応する著者

研究成果: Article査読

13 被引用数 (Scopus)

抄録

Intrinsic and lipid phase transition-induced conformational changes in cytochrome oxidase in phosphatidylcholine vesicle and solubilized systems were examined by the fluorescence lifetime of N-(1-anilinonaphthyl-4)-maleimide conjugated with the enzyme. The time-dependent fluorescence intensity of N-(1-anilinonaphthyl-4)-maleimide attached to cytochrome oxidase was described as a triple exponential decay. Both the intrinsic and lipid phase transition-induced conformational changes were detectable in plots of the average lifetime against temperature. In most cases a peak occurred at the temperature of the conformational change. The time-dependent emission anisotropy showed that N-(1-anilinonaphthyl-4)-maleimide embedded in cytochrome oxidase in phosphatidylcholine vesicles underwent a rapid restricted wobbling within a cone. The half-angle of the cone was around 30° for cytochrome oxidase in dimyristoyl phosphatidylcholine vesicles.

本文言語English
ページ(範囲)85-92
ページ数8
ジャーナルBBA - Bioenergetics
634
C
DOI
出版ステータスPublished - 1981
外部発表はい

ASJC Scopus subject areas

  • 生物理学
  • 医学(全般)

フィンガープリント

「Nanosecond time-resolved fluorescence investigations of temperature-induced conformational changes in cytochrome oxidase in phosphatidylcholine vesicles and solubilized systems」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル