Nanosecond time-resolved fluorescence investigations of temperature-induced conformational changes in cytochrome oxidase in phosphatidylcholine vesicles and solubilized systems

Suguru Kawato, Satoshi Yoshida, Yutaka Orii, Akira Ikegami, Kazuhiko Kinosita

研究成果: Article

13 引用 (Scopus)

抄録

Intrinsic and lipid phase transition-induced conformational changes in cytochrome oxidase in phosphatidylcholine vesicle and solubilized systems were examined by the fluorescence lifetime of N-(1-anilinonaphthyl-4)-maleimide conjugated with the enzyme. The time-dependent fluorescence intensity of N-(1-anilinonaphthyl-4)-maleimide attached to cytochrome oxidase was described as a triple exponential decay. Both the intrinsic and lipid phase transition-induced conformational changes were detectable in plots of the average lifetime against temperature. In most cases a peak occurred at the temperature of the conformational change. The time-dependent emission anisotropy showed that N-(1-anilinonaphthyl-4)-maleimide embedded in cytochrome oxidase in phosphatidylcholine vesicles underwent a rapid restricted wobbling within a cone. The half-angle of the cone was around 30° for cytochrome oxidase in dimyristoyl phosphatidylcholine vesicles.

元の言語English
ページ(範囲)85-92
ページ数8
ジャーナルBBA - Bioenergetics
634
発行部数C
DOI
出版物ステータスPublished - 1981
外部発表Yes

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Electron Transport Complex IV
Phosphatidylcholines
Fluorescence
Temperature
Phase Transition
Cones
Phase transitions
Lipids
Anisotropy
Enzymes
N-(1-anilinonaphthalyl)-4-maleimide

ASJC Scopus subject areas

  • Biophysics
  • Medicine(all)

これを引用

Nanosecond time-resolved fluorescence investigations of temperature-induced conformational changes in cytochrome oxidase in phosphatidylcholine vesicles and solubilized systems. / Kawato, Suguru; Yoshida, Satoshi; Orii, Yutaka; Ikegami, Akira; Kinosita, Kazuhiko.

:: BBA - Bioenergetics, 巻 634, 番号 C, 1981, p. 85-92.

研究成果: Article

Kawato, Suguru ; Yoshida, Satoshi ; Orii, Yutaka ; Ikegami, Akira ; Kinosita, Kazuhiko. / Nanosecond time-resolved fluorescence investigations of temperature-induced conformational changes in cytochrome oxidase in phosphatidylcholine vesicles and solubilized systems. :: BBA - Bioenergetics. 1981 ; 巻 634, 番号 C. pp. 85-92.
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abstract = "Intrinsic and lipid phase transition-induced conformational changes in cytochrome oxidase in phosphatidylcholine vesicle and solubilized systems were examined by the fluorescence lifetime of N-(1-anilinonaphthyl-4)-maleimide conjugated with the enzyme. The time-dependent fluorescence intensity of N-(1-anilinonaphthyl-4)-maleimide attached to cytochrome oxidase was described as a triple exponential decay. Both the intrinsic and lipid phase transition-induced conformational changes were detectable in plots of the average lifetime against temperature. In most cases a peak occurred at the temperature of the conformational change. The time-dependent emission anisotropy showed that N-(1-anilinonaphthyl-4)-maleimide embedded in cytochrome oxidase in phosphatidylcholine vesicles underwent a rapid restricted wobbling within a cone. The half-angle of the cone was around 30° for cytochrome oxidase in dimyristoyl phosphatidylcholine vesicles.",
keywords = "(Nanosecond spectroscopy), Conformational change, Cytochrome oxidase, Fluorescence anisotropy, Phase transition, Phosphatidylcholine vesicle",
author = "Suguru Kawato and Satoshi Yoshida and Yutaka Orii and Akira Ikegami and Kazuhiko Kinosita",
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T1 - Nanosecond time-resolved fluorescence investigations of temperature-induced conformational changes in cytochrome oxidase in phosphatidylcholine vesicles and solubilized systems

AU - Kawato, Suguru

AU - Yoshida, Satoshi

AU - Orii, Yutaka

AU - Ikegami, Akira

AU - Kinosita, Kazuhiko

PY - 1981

Y1 - 1981

N2 - Intrinsic and lipid phase transition-induced conformational changes in cytochrome oxidase in phosphatidylcholine vesicle and solubilized systems were examined by the fluorescence lifetime of N-(1-anilinonaphthyl-4)-maleimide conjugated with the enzyme. The time-dependent fluorescence intensity of N-(1-anilinonaphthyl-4)-maleimide attached to cytochrome oxidase was described as a triple exponential decay. Both the intrinsic and lipid phase transition-induced conformational changes were detectable in plots of the average lifetime against temperature. In most cases a peak occurred at the temperature of the conformational change. The time-dependent emission anisotropy showed that N-(1-anilinonaphthyl-4)-maleimide embedded in cytochrome oxidase in phosphatidylcholine vesicles underwent a rapid restricted wobbling within a cone. The half-angle of the cone was around 30° for cytochrome oxidase in dimyristoyl phosphatidylcholine vesicles.

AB - Intrinsic and lipid phase transition-induced conformational changes in cytochrome oxidase in phosphatidylcholine vesicle and solubilized systems were examined by the fluorescence lifetime of N-(1-anilinonaphthyl-4)-maleimide conjugated with the enzyme. The time-dependent fluorescence intensity of N-(1-anilinonaphthyl-4)-maleimide attached to cytochrome oxidase was described as a triple exponential decay. Both the intrinsic and lipid phase transition-induced conformational changes were detectable in plots of the average lifetime against temperature. In most cases a peak occurred at the temperature of the conformational change. The time-dependent emission anisotropy showed that N-(1-anilinonaphthyl-4)-maleimide embedded in cytochrome oxidase in phosphatidylcholine vesicles underwent a rapid restricted wobbling within a cone. The half-angle of the cone was around 30° for cytochrome oxidase in dimyristoyl phosphatidylcholine vesicles.

KW - (Nanosecond spectroscopy)

KW - Conformational change

KW - Cytochrome oxidase

KW - Fluorescence anisotropy

KW - Phase transition

KW - Phosphatidylcholine vesicle

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JO - Biochimica et Biophysica Acta - Bioenergetics

JF - Biochimica et Biophysica Acta - Bioenergetics

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