Nitrite oxidation kinetics of two Nitrospira strains: The quest for competition and ecological niche differentiation

Norisuke Ushiki, Masaru Jinno, Hirotsugu Fujitani, Toshikazu Suenaga, Akihiko Terada, Satoshi Tsuneda

研究成果: Article

27 引用 (Scopus)

抄録

Nitrite oxidation is an aerobic process of the nitrogen cycle in natural ecosystems, and is performed by nitrite-oxidizing bacteria (NOB). Also, nitrite oxidation is a rate-limiting step of nitrogen removal in wastewater treatment plants (WWTPs). Although Nitrospira is known as dominant NOB in WWTPs, information on their physiological properties and kinetic parameters is limited. Here, we report the kinetic parameters and inhibition of nitrite oxidation by free ammonia in pure cultures of Nitrospira sp. strain ND1 and Nitrospira japonica strain NJ1, which were previously isolated from activated sludge in a WWTP. The maximum nitrite uptake rate (Vmax_NO2) and the half-saturation constant for nitrite uptake (Km_NO2) of strains ND1 and NJ1 were 45 ± 7 and 31 ± 5 (μmol NO2 -/mg protein/h), and 6 ± 1 and 10 ± 2 (μM NO2 -), respectively. The Vmax_NO2 and Km_NO2 of two strains indicated that they adapt to low-nitrite-concentration environments like activated sludge. The half-saturation constants for oxygen uptake (Km_O2) of the two strains were 4.0±2.5 and 2.6±1.1 (μM O2), respectively. The Km_O2 values of the two strains were lower than those of other NOB, suggesting that Nitrospira in activated sludge could oxidize nitrite in the hypoxic environments often found in the interiors of biofilms and flocs. The inhibition thresholds of the two strains by free ammonia were 0.85 and 4.3 (mg-NH3 l-1), respectively. Comparing the physiological properties of the two strains, we suggest that tolerance for free ammonia determines competition and partitioning into ecological niches among Nitrospira populations.

元の言語English
ジャーナルJournal of Bioscience and Bioengineering
DOI
出版物ステータスAccepted/In press - 2016 11 28

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Nitrites
Wastewater treatment
Ammonia
Bacteria
Kinetic parameters
Oxidation
Kinetics
Nitrogen removal
Biofilms
Ecosystems
Waste Water
Sewage
Nitrogen
Proteins
Oxygen
Nitrogen Cycle
Ecosystem

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

これを引用

Nitrite oxidation kinetics of two Nitrospira strains : The quest for competition and ecological niche differentiation. / Ushiki, Norisuke; Jinno, Masaru; Fujitani, Hirotsugu; Suenaga, Toshikazu; Terada, Akihiko; Tsuneda, Satoshi.

:: Journal of Bioscience and Bioengineering, 28.11.2016.

研究成果: Article

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abstract = "Nitrite oxidation is an aerobic process of the nitrogen cycle in natural ecosystems, and is performed by nitrite-oxidizing bacteria (NOB). Also, nitrite oxidation is a rate-limiting step of nitrogen removal in wastewater treatment plants (WWTPs). Although Nitrospira is known as dominant NOB in WWTPs, information on their physiological properties and kinetic parameters is limited. Here, we report the kinetic parameters and inhibition of nitrite oxidation by free ammonia in pure cultures of Nitrospira sp. strain ND1 and Nitrospira japonica strain NJ1, which were previously isolated from activated sludge in a WWTP. The maximum nitrite uptake rate (Vmax_NO2) and the half-saturation constant for nitrite uptake (Km_NO2) of strains ND1 and NJ1 were 45 ± 7 and 31 ± 5 (μmol NO2 -/mg protein/h), and 6 ± 1 and 10 ± 2 (μM NO2 -), respectively. The Vmax_NO2 and Km_NO2 of two strains indicated that they adapt to low-nitrite-concentration environments like activated sludge. The half-saturation constants for oxygen uptake (Km_O2) of the two strains were 4.0±2.5 and 2.6±1.1 (μM O2), respectively. The Km_O2 values of the two strains were lower than those of other NOB, suggesting that Nitrospira in activated sludge could oxidize nitrite in the hypoxic environments often found in the interiors of biofilms and flocs. The inhibition thresholds of the two strains by free ammonia were 0.85 and 4.3 (mg-NH3 l-1), respectively. Comparing the physiological properties of the two strains, we suggest that tolerance for free ammonia determines competition and partitioning into ecological niches among Nitrospira populations.",
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T2 - The quest for competition and ecological niche differentiation

AU - Ushiki, Norisuke

AU - Jinno, Masaru

AU - Fujitani, Hirotsugu

AU - Suenaga, Toshikazu

AU - Terada, Akihiko

AU - Tsuneda, Satoshi

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N2 - Nitrite oxidation is an aerobic process of the nitrogen cycle in natural ecosystems, and is performed by nitrite-oxidizing bacteria (NOB). Also, nitrite oxidation is a rate-limiting step of nitrogen removal in wastewater treatment plants (WWTPs). Although Nitrospira is known as dominant NOB in WWTPs, information on their physiological properties and kinetic parameters is limited. Here, we report the kinetic parameters and inhibition of nitrite oxidation by free ammonia in pure cultures of Nitrospira sp. strain ND1 and Nitrospira japonica strain NJ1, which were previously isolated from activated sludge in a WWTP. The maximum nitrite uptake rate (Vmax_NO2) and the half-saturation constant for nitrite uptake (Km_NO2) of strains ND1 and NJ1 were 45 ± 7 and 31 ± 5 (μmol NO2 -/mg protein/h), and 6 ± 1 and 10 ± 2 (μM NO2 -), respectively. The Vmax_NO2 and Km_NO2 of two strains indicated that they adapt to low-nitrite-concentration environments like activated sludge. The half-saturation constants for oxygen uptake (Km_O2) of the two strains were 4.0±2.5 and 2.6±1.1 (μM O2), respectively. The Km_O2 values of the two strains were lower than those of other NOB, suggesting that Nitrospira in activated sludge could oxidize nitrite in the hypoxic environments often found in the interiors of biofilms and flocs. The inhibition thresholds of the two strains by free ammonia were 0.85 and 4.3 (mg-NH3 l-1), respectively. Comparing the physiological properties of the two strains, we suggest that tolerance for free ammonia determines competition and partitioning into ecological niches among Nitrospira populations.

AB - Nitrite oxidation is an aerobic process of the nitrogen cycle in natural ecosystems, and is performed by nitrite-oxidizing bacteria (NOB). Also, nitrite oxidation is a rate-limiting step of nitrogen removal in wastewater treatment plants (WWTPs). Although Nitrospira is known as dominant NOB in WWTPs, information on their physiological properties and kinetic parameters is limited. Here, we report the kinetic parameters and inhibition of nitrite oxidation by free ammonia in pure cultures of Nitrospira sp. strain ND1 and Nitrospira japonica strain NJ1, which were previously isolated from activated sludge in a WWTP. The maximum nitrite uptake rate (Vmax_NO2) and the half-saturation constant for nitrite uptake (Km_NO2) of strains ND1 and NJ1 were 45 ± 7 and 31 ± 5 (μmol NO2 -/mg protein/h), and 6 ± 1 and 10 ± 2 (μM NO2 -), respectively. The Vmax_NO2 and Km_NO2 of two strains indicated that they adapt to low-nitrite-concentration environments like activated sludge. The half-saturation constants for oxygen uptake (Km_O2) of the two strains were 4.0±2.5 and 2.6±1.1 (μM O2), respectively. The Km_O2 values of the two strains were lower than those of other NOB, suggesting that Nitrospira in activated sludge could oxidize nitrite in the hypoxic environments often found in the interiors of biofilms and flocs. The inhibition thresholds of the two strains by free ammonia were 0.85 and 4.3 (mg-NH3 l-1), respectively. Comparing the physiological properties of the two strains, we suggest that tolerance for free ammonia determines competition and partitioning into ecological niches among Nitrospira populations.

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KW - Affinity for oxygen

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KW - Growth parameters

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