Nitrogen fixation by immobilized Azotobacter chroococcum

Isao Karube, Tadashi Matsunaga, Yasuo Otomine, Shuichi Suzuki

研究成果: Article

6 引用 (Scopus)

抄録

A nitrogen-fixing bacterium, Azotobacter chroococcum, was immobilized in 2% agar gel. The optimum partial oxygen pressure, pO2, of immobilized cells was 0.2 atm, wherea s that of native cells was 0.05 atm. When continual nitrogen fixation was performed under aerobic conditions, the nitrogenase activity of immobilized cells increased with increasing time. On the other hand, the activity of native cells decreased rapidly. Increase of nitrogenase activity was attributed to growth of the bacteria in the gel matrix. The production rate of total nitrogen compounds by the immobilized bacteria was also increased during the first 4 days. Nitrogen compounds produced by the immobilized cells were mainly amino acids such as γ-aminobutyrate, glutamate and arginine.

元の言語English
ページ(範囲)309-312
ページ数4
ジャーナルEnzyme and Microbial Technology
3
発行部数4
DOI
出版物ステータスPublished - 1981
外部発表Yes

Fingerprint

Azotobacter
Nitrogen fixation
Immobilized Cells
Nitrogen Fixation
Nitrogen Compounds
Nitrogenase
Cells
arginine glutamate
Nitrogen compounds
Bacteria
Gels
Aminobutyrates
Partial Pressure
Arginine
Agar
Oxygen
Amino acids
Amino Acids
Nitrogen
Growth

ASJC Scopus subject areas

  • Biochemistry
  • Biotechnology
  • Applied Microbiology and Biotechnology

これを引用

Nitrogen fixation by immobilized Azotobacter chroococcum. / Karube, Isao; Matsunaga, Tadashi; Otomine, Yasuo; Suzuki, Shuichi.

:: Enzyme and Microbial Technology, 巻 3, 番号 4, 1981, p. 309-312.

研究成果: Article

Karube, I, Matsunaga, T, Otomine, Y & Suzuki, S 1981, 'Nitrogen fixation by immobilized Azotobacter chroococcum', Enzyme and Microbial Technology, 巻. 3, 番号 4, pp. 309-312. https://doi.org/10.1016/0141-0229(81)90005-3
Karube, Isao ; Matsunaga, Tadashi ; Otomine, Yasuo ; Suzuki, Shuichi. / Nitrogen fixation by immobilized Azotobacter chroococcum. :: Enzyme and Microbial Technology. 1981 ; 巻 3, 番号 4. pp. 309-312.
@article{070eb57e7f5e4203b91129c63c710dac,
title = "Nitrogen fixation by immobilized Azotobacter chroococcum",
abstract = "A nitrogen-fixing bacterium, Azotobacter chroococcum, was immobilized in 2{\%} agar gel. The optimum partial oxygen pressure, pO2, of immobilized cells was 0.2 atm, wherea s that of native cells was 0.05 atm. When continual nitrogen fixation was performed under aerobic conditions, the nitrogenase activity of immobilized cells increased with increasing time. On the other hand, the activity of native cells decreased rapidly. Increase of nitrogenase activity was attributed to growth of the bacteria in the gel matrix. The production rate of total nitrogen compounds by the immobilized bacteria was also increased during the first 4 days. Nitrogen compounds produced by the immobilized cells were mainly amino acids such as γ-aminobutyrate, glutamate and arginine.",
author = "Isao Karube and Tadashi Matsunaga and Yasuo Otomine and Shuichi Suzuki",
year = "1981",
doi = "10.1016/0141-0229(81)90005-3",
language = "English",
volume = "3",
pages = "309--312",
journal = "Enzyme and Microbial Technology",
issn = "0141-0229",
publisher = "Elsevier Inc.",
number = "4",

}

TY - JOUR

T1 - Nitrogen fixation by immobilized Azotobacter chroococcum

AU - Karube, Isao

AU - Matsunaga, Tadashi

AU - Otomine, Yasuo

AU - Suzuki, Shuichi

PY - 1981

Y1 - 1981

N2 - A nitrogen-fixing bacterium, Azotobacter chroococcum, was immobilized in 2% agar gel. The optimum partial oxygen pressure, pO2, of immobilized cells was 0.2 atm, wherea s that of native cells was 0.05 atm. When continual nitrogen fixation was performed under aerobic conditions, the nitrogenase activity of immobilized cells increased with increasing time. On the other hand, the activity of native cells decreased rapidly. Increase of nitrogenase activity was attributed to growth of the bacteria in the gel matrix. The production rate of total nitrogen compounds by the immobilized bacteria was also increased during the first 4 days. Nitrogen compounds produced by the immobilized cells were mainly amino acids such as γ-aminobutyrate, glutamate and arginine.

AB - A nitrogen-fixing bacterium, Azotobacter chroococcum, was immobilized in 2% agar gel. The optimum partial oxygen pressure, pO2, of immobilized cells was 0.2 atm, wherea s that of native cells was 0.05 atm. When continual nitrogen fixation was performed under aerobic conditions, the nitrogenase activity of immobilized cells increased with increasing time. On the other hand, the activity of native cells decreased rapidly. Increase of nitrogenase activity was attributed to growth of the bacteria in the gel matrix. The production rate of total nitrogen compounds by the immobilized bacteria was also increased during the first 4 days. Nitrogen compounds produced by the immobilized cells were mainly amino acids such as γ-aminobutyrate, glutamate and arginine.

UR - http://www.scopus.com/inward/record.url?scp=0019624844&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019624844&partnerID=8YFLogxK

U2 - 10.1016/0141-0229(81)90005-3

DO - 10.1016/0141-0229(81)90005-3

M3 - Article

AN - SCOPUS:0019624844

VL - 3

SP - 309

EP - 312

JO - Enzyme and Microbial Technology

JF - Enzyme and Microbial Technology

SN - 0141-0229

IS - 4

ER -