No transcription-translation feedback in circadian rhythm of KaiC phosphorylation

Jun Tomita, Masato Nakajima, Takao Kondo, Hideo Iwasaki*

*この研究の対応する著者

研究成果: Article査読

358 被引用数 (Scopus)

抄録

An autoregulatory transcription-translation feedback loop is thought to be essential in generating circadian rhythms in any model organism. In the cyanobacterium Synechococcus elongatus, the essential clock protein KaiC is proposed to form this type of transcriptional negative feedback. Nevertheless, we demonstrate here temperature-compensated, robust circadian cycling of KaiC phosphorylation even without kaiBC messenger RNA accumulation under continuous dark conditions. This rhythm persisted in the presence of a transcription or translation inhibitor. Moreover, kinetic profiles in the ratio of KaiC autophosphorylation-dephosphorylation were also temperature compensated in vitro. Thus, the cyanobacterial clock can keep time independent of de novo transcription and translation processes.

本文言語English
ページ(範囲)251-254
ページ数4
ジャーナルScience
307
5707
DOI
出版ステータスPublished - 2005 1 14
外部発表はい

ASJC Scopus subject areas

  • 一般

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