抄録
To investigate the non-genetic variability of the division cycle and growth of single cells, we compared pairs of Escherichia coli daughter cells' growth and division time under isolated conditions using a newly developed on-chip culture system. The advantages of the system are: (i) continuous cultivation of isolated single cells or a group of cells in μm-sized micro-cages under isolated contamination-free and exchangeable medium conditions, and (ii) continuous observation and comparison of those floating daughter cells at a spatial resolution of 0.2 μm by phase-contrast/fluorescence microscopy and digital image processing. Using this system, cell growth and division time of daughter cells from an isolated mother cell were measured. A broad distribution of the cell division time and the division time differences of two daughter cells from the same mother cells were found. They are not attributable to a difference in DNA. The same tendency of broad distribution of the division time and the division time differences suggests the involvement of a probabilistic process to start the division.
| 本文言語 | English |
|---|---|
| ページ（範囲） | 145-150 |
| ページ数 | 6 |
| ジャーナル | Proceedings of the Japan Academy Series B: Physical and Biological Sciences |
| 巻 | 77 |
| 号 | 8 |
| DOI | |
| 出版ステータス | Published - 2001 10月 |
| 外部発表 | はい |
ASJC Scopus subject areas
- 農業および生物科学（全般）
- 物理学および天文学（全般）