Non-genetic variability of division cycle and growth of isolated individual cells in on-chip culture system

Ippei Inoue, Yuichi Wakamoto, Kenji Yasuda*

*この研究の対応する著者

研究成果: Article査読

28 被引用数 (Scopus)

抄録

To investigate the non-genetic variability of the division cycle and growth of single cells, we compared pairs of Escherichia coli daughter cells' growth and division time under isolated conditions using a newly developed on-chip culture system. The advantages of the system are: (i) continuous cultivation of isolated single cells or a group of cells in μm-sized micro-cages under isolated contamination-free and exchangeable medium conditions, and (ii) continuous observation and comparison of those floating daughter cells at a spatial resolution of 0.2 μm by phase-contrast/fluorescence microscopy and digital image processing. Using this system, cell growth and division time of daughter cells from an isolated mother cell were measured. A broad distribution of the cell division time and the division time differences of two daughter cells from the same mother cells were found. They are not attributable to a difference in DNA. The same tendency of broad distribution of the division time and the division time differences suggests the involvement of a probabilistic process to start the division.

本文言語English
ページ(範囲)145-150
ページ数6
ジャーナルProceedings of the Japan Academy Series B: Physical and Biological Sciences
77
8
DOI
出版ステータスPublished - 2001 10
外部発表はい

ASJC Scopus subject areas

  • 農業および生物科学(全般)
  • 物理学および天文学(全般)

フィンガープリント

「Non-genetic variability of division cycle and growth of isolated individual cells in on-chip culture system」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル