Novel detection system for biomolecules using nano-sized bacterial magnetic particles and magnetic force microscopy

Yosuke Amemiya, Tsuyoshi Tanaka, Brandon Yoza, Tadashi Matsunaga

研究成果: Article

45 引用 (Scopus)

抄録

A system for streptavidin detection using biotin conjugated to nano-sized bacterial magnetic particles (BMPs) has been developed. BMPs, isolated from magnetic bacteria, were used as magnetic markers for magnetic force microscopy (MFM) imaging. The magnetic signal was obtained from a single particle using MFM without application of an external magnetic field. The number of biotin conjugated BMPs (biotin-BMPs) bound to streptavidin immobilized on the glass slides increased with streptavidin concentrations up to 100 pg/ml. The minimum streptavidin detection limit using this technique is 1 pg/ml, which is 100 times more sensitive than a conventional fluorescent detection system. This is the first report using single domain nano-sized magnetic particles as magnetic markers for biosensing. This assay system can be used for immunoassay and DNA detection with high sensitivities.

元の言語English
ページ(範囲)308-314
ページ数7
ジャーナルJournal of Biotechnology
120
発行部数3
DOI
出版物ステータスPublished - 2005 11 21
外部発表Yes

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Magnetic force microscopy
Streptavidin
Atomic Force Microscopy
Biomolecules
Biotin
Assays
Bacteria
DNA
Magnetic fields
Imaging techniques
Glass
Magnetic Fields
Immunoassay
Limit of Detection

ASJC Scopus subject areas

  • Biotechnology

これを引用

Novel detection system for biomolecules using nano-sized bacterial magnetic particles and magnetic force microscopy. / Amemiya, Yosuke; Tanaka, Tsuyoshi; Yoza, Brandon; Matsunaga, Tadashi.

:: Journal of Biotechnology, 巻 120, 番号 3, 21.11.2005, p. 308-314.

研究成果: Article

Amemiya, Yosuke ; Tanaka, Tsuyoshi ; Yoza, Brandon ; Matsunaga, Tadashi. / Novel detection system for biomolecules using nano-sized bacterial magnetic particles and magnetic force microscopy. :: Journal of Biotechnology. 2005 ; 巻 120, 番号 3. pp. 308-314.
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