Novel fish hypothalamic neuropeptides stimulate the release of gonadotrophins and growth hormone from the pituitary of sockeye salmon

Masafumi Amano, Shunsuke Moriyama, Masayuki Ligo, Shoji Kitamura, Noriko Amiya, Kunio Yamamori, Kazuyoshi Ukena, Kazuyoshi Tsutsui*

*この研究の対応する著者

研究成果: Article査読

110 被引用数 (Scopus)

抄録

We recently identified a cDNA encoding three novel fish hypothalamic neuropeptides, having LPXRF-NH2 from the goldfish brain. In this study, to clarify the physiological functions of these three LPXRFamide peptides (gfLPXRFa-1, -2, and -3), we analysed the localisation and hypophysiotrophic activity of these peptides using sockeye salmon, Oncorhynchus nerka, in which immunoassay systems for several anterior pituitary hormones have been developed. gfLPXRFa-immunoreactive cell bodies were detected in the nucleus posterioris periventricularis of the hypothalamus and immunoreactive fibres were distributed in various brain regions and the pituitary. We also detected gfLPXRFa-immunoreactivity in the pituitary by competitive enzyme-linked immunosorbent assay combined with reversed-phase HPLC. These three gfLPXRFamide peptides stimulated the release of FSH, LH and GH, but did not affect the release of prolactin (PRL) and somatolactin (SL) from cultured pituitary cells. These results suggest that novel fish hypothalamic LPXR-Famide peptides exist in the brain and pituitary of sockeye salmon and stimulate the release of gonadotrophins and GH from the pituitary.

本文言語English
ページ(範囲)417-423
ページ数7
ジャーナルJournal of Endocrinology
188
3
DOI
出版ステータスPublished - 2006 3月
外部発表はい

ASJC Scopus subject areas

  • 内分泌学

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