Novel monofunctional substrates of polynucleotide phosphorylase: The "single-addition" of 2′(3′)-o-dihydrocinnamoyl-nucleoside 5′-diphosphate to a primer oligonucleotide

Yo Kikuchi*, Keiko Hirai, Kenji Sakaguchi

*この研究の対応する著者

研究成果: Article査読

15 被引用数 (Scopus)

抄録

A method was developed for stepwise synthesis of oligonucleotides of denned sequence using 2′(3′)-O-dihydrocinnamoyl-nucleoside 5′-diphosphates as substrates for polynucleotide phosphorylase [EC 2.7.7.8]. Polynucleotide phosphorylase from Thermus thermophilus catalyzed the transfer of one 2′(3′)-O-dihydrocinnamoyl-adenylate residue from 2′(3′)-blocked ADP to the 3′-terminus of the primer trinucleoside diphosphate, ApApA. The product was 2′(3′)-substituted triadenylyladenosine. The blocking group, dihydrocinnamoyl, could be removed completely from the product without destruction of the phosphodiester bond using α-chymotrypsin [EC 3.4.21.1] at neutral pH.

本文言語English
ページ(範囲)469-472
ページ数4
ジャーナルJournal of Biochemistry
77
2
出版ステータスPublished - 1975 2
外部発表はい

ASJC Scopus subject areas

  • 統計学、確率および不確実性
  • 応用数学
  • 生理学(医学)
  • 放射線学、核医学およびイメージング
  • 分子生物学
  • 生化学

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