Novel myosin heavy chain kinase involved in disassembly of myosin II filaments and efficient cleavage in mitotic Dictyostelium cells

Akira Nagasaki*, Go Itoh, Shigehiko Yumura, Taro Q.P. Uyeda

*この研究の対応する著者

研究成果: Article査読

23 被引用数 (Scopus)

抄録

We have cloned a full-length cDNA encoding a novel myosin II heavy chain kinase (mhckC) from Dictyostelium. Like other members of the myosin heavy chain kinase family, the mhckC gene product, MHCK C, has a kinase domain in its N-terminal half and six WD repeats in the C-terminal half. GFP-MHCK C fusion protein localized to the cortex of interphase cells, to the cleavage furrow of mitotic cells, and to the posterior of migrating cells. These distributions of GFP-MHCK C always corresponded with that of myosin II filaments and were not observed in myosin II-null cells, where GFP-MHCK C was diffusely distributed in the cytoplasm. Thus, localization of MHCK C seems to be myosin II-dependent. Cells lacking the mhckC gene exhibited excessive aggregation of myosin II filaments in the cleavage furrows and in the posteriors of the daughter cells once cleavage was complete. The cleavage process of these cells took longer than that of wild-type cells. Taken together, these findings suggest MHCK C drives the disassembly of myosin II filaments for efficient cytokinesis and recycling of myosin II that occurs during cytokinesis.

本文言語English
ページ(範囲)4333-4342
ページ数10
ジャーナルMolecular biology of the cell
13
12
DOI
出版ステータスPublished - 2002 12 1
外部発表はい

ASJC Scopus subject areas

  • 分子生物学
  • 細胞生物学

フィンガープリント

「Novel myosin heavy chain kinase involved in disassembly of myosin II filaments and efficient cleavage in mitotic Dictyostelium cells」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル