Novel temperature-responsive polymer brushes with carbohydrate residues facilitate selective adhesion and collection of hepatocytes

Naokazu Idota*, Mitsuhiro Ebara, Yohei Kotsuchibashi, Ravin Narain, Takao Aoyagi

*この研究の対応する著者

研究成果: Article査読

32 被引用数 (Scopus)

抄録

Temperature-responsive glycopolymer brushes were designed to investigate the effects of grafting architectures of the copolymers on the selective adhesion and collection of hypatocytes. Homo, random and block sequences of N-isopropylacrylamide and 2-lactobionamidoethyl methacrylate were grafted on glass substrates via surface-initiated atom transfer radical polymerization. The galactose/lactose-specific lectin RCA120 and HepG2 cells were used to test for specific recognition of the polymer brushes containing galactose residues over the lower critical solution temperatures (LCSTs). RCA 120 showed a specific binding to the brush surfaces at 37 °C. These brush surfaces also facilitated the adhesion of HepG2 cells at 37 °C under nonserum conditions, whereas no adhesion was observed for NIH-3T3 fibroblasts. When the temperature was decreased to 25 °C, almost all the HepG2 cells detached from the block copolymer brush, whereas the random copolymer brush did not release the cells. The difference in releasing kinetics of cells from the surfaces with different grafting architectures can be explained by the correlated effects of significant changes in LCST, mobility, hydrophilicity and mechanical properties of the grafted polymer chains. These findings are important for designing 'on-off' cell capture/release substrates for various biomedical applications such as selective cell separation.

本文言語English
論文番号064206
ジャーナルScience and Technology of Advanced Materials
13
6
DOI
出版ステータスPublished - 2012 12月
外部発表はい

ASJC Scopus subject areas

  • 材料科学(全般)

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