Nuclear PP2A-Cdc55 prevents APC-Cdc20 activation during the spindle assembly checkpoint

Valentina Rossio, Takeshi Michimoto, Takeshi Sasaki, Iwai Ohbayashi, Yoshiko Kikuchi, Satoshi Yoshida*

*この研究の対応する著者

研究成果: Article査読

9 被引用数 (Scopus)

抄録

Cdc55, a regulatory B-subunit of protein phosphatase 2A (PP2A) complex, is essential for the spindle assembly checkpoint (SAC) in budding yeast, but the regulation and molecular targets of PP2A-Cdc55 have not been clearly defined or are controversial. Here, we show that an important target of Cdc55 in the SAC is the anaphase-promoting complex (APC) coupled with Cdc20 and that APC-Cdc20 is kept inactive by dephosphorylation by nuclear PP2A-Cdc55 when spindle is damaged. By isolating a new class of Cdc55 mutants specifically defective in the SAC and by artificially manipulating nucleocytoplasmic distribution of Cdc55, we further show that nuclear Cdc55 is essential for the SAC. Because the Cdc55-binding proteins Zds1 and Zds2 inhibit both nuclear accumulation of Cdc55 and SAC activity, we propose that spatial control of PP2A by Zds1 family proteins is important for tight control of SAC and mitotic progression.

本文言語English
ページ(範囲)4396-4405
ページ数10
ジャーナルJournal of Cell Science
126
19
DOI
出版ステータスPublished - 2013
外部発表はい

ASJC Scopus subject areas

  • 細胞生物学

フィンガープリント

「Nuclear PP2A-Cdc55 prevents APC-Cdc20 activation during the spindle assembly checkpoint」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル