@article{8338e4134c2c4e02bdf2dbb305e36138,
title = "Nuclear respiratory factor 2 activates transcription of human mitochondrial translation initiation factor 2 gene",
abstract = "We studied the transcriptional regulation of the human mitochondrial translation initiation factor 2 (IF2mt) gene. The minimal promoter region for the human IF2mt gene contains binding sites for Nuclear Respiratory Factor 2 (NRF-2), which is often involved in the transcription of mitochondrial-related genes. Electrophoresis mobility shift assay (EMSA) analyses indicated that NRF-2α/β binds to the IF2mt promoter. Reporter assays, where HEK293T cells were co-transfected with an NRF-2α/β-expressing vector and/or an IF2mt promoter reporter vector, revealed that NRF-2 trans-activates the IF2mt promoter. NRF-2 sites were also found in the promoters of several other mitochondrial translation factors, which suggests NRF-2 may play a key role in the regulation of mitochondrial protein synthesis.",
keywords = "Initiation factor 2, Mitochondria, Nuclear respiratory factor 2, Transcriptional regulation, Translation",
author = "Rippei Hayashi and Takuya Ueda and Farwell, {Mary A.} and Nono Takeuchi",
note = "Funding Information: We thank Dr. Hiroshi Handa (Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Japan) for the kind gift of pGEX-NRF-2β and Dr. Richard C. Scarpulla (Department of Cell and Molecular Biology, Northwestern University Medical School, Chicago, Illinois, U.S.A.) for the kind gift of NRF-2α antiserum. We are also grateful to Dr. Yasunori Miyamoto (Department of Biology, Ochanomizu University, Tokyo, Japan) for helpful suggestions about the EMSA analysis. This work was supported by a Japan Society Promotion of Science Fellowship for Japanese Junior Scientists to Rippei Hayashi and by a grant from the Ministry of Education, Culture, Sports, Science, and Technology of Japan to Nono Takeuchi.",
year = "2007",
month = may,
doi = "10.1016/j.mito.2006.10.005",
language = "English",
volume = "7",
pages = "195--203",
journal = "Mitochondrion",
issn = "1567-7249",
publisher = "Elsevier",
number = "3",
}