Nucleocytoplasmic transport of Alp7/TACC organizes spatiotemporal microtubule formation in fission yeast

Masamitsu Sato*, Naoyuki Okada, Yasutaka Kakui, Masayuki Yamamoto, Minoru Yoshida, Takashi Toda

*この研究の対応する著者

研究成果: Article査読

24 被引用数 (Scopus)

抄録

Ran GTPase activates several target molecules to induce microtubule formation around the chromosomes and centrosomes. In fission yeast, in which the nuclear envelope does not break down during mitosis, Ran targets the centrosomal transforming acidic coiled-coil (TACC) protein Alp7 for spindle formation. Alp7 accumulates in the nucleus only during mitosis, although its underlying mechanism remains elusive. Here, we investigate the behaviour of Alp7 and its binding partner, Alp14/TOG, throughout the cell cycle. Interestingly, Alp7 enters the nucleus during interphase but is subsequently exported to the cytoplasm by the Exportin-dependent nuclear export machinery. The continuous nuclear export of Alp7 during interphase is essential for maintaining the array-like cytoplasmic microtubule structure. The mitosis-specific nuclear accumulation of Alp7 seems to be under the control of cyclin-dependent kinase (CDK). These results indicate that the spatiotemporal regulation of microtubule formation is established by the Alp7/TACC-Alp14/TOG complex through the coordinated interplay of Ran and CDK.

本文言語English
ページ(範囲)1161-1167
ページ数7
ジャーナルEMBO Reports
10
10
DOI
出版ステータスPublished - 2009
外部発表はい

ASJC Scopus subject areas

  • 生化学
  • 分子生物学
  • 遺伝学

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