Nucleotide binding and autophosphorylation of the clock protein KaiC as a circadian timing process of cyanobacteria

Taeko Nishiwaki, Hideo Iwasaki, Masahiro Ishiura, Takao Kondo*

*この研究の対応する著者

研究成果: Article査読

150 被引用数 (Scopus)

抄録

A negative feedback control of kaiC expression by KaiC protein has been proposed to generate a basic oscillation of the circadian clock in the cyanobacterium Synechococcus sp. PCC 7942. KaiC has two P loops or Walker's motif As, that are potential ATP-/GTP-binding motifs and DXXG motifs conserved in various GTP-binding proteins. Herein, we demonstrate that in vitro KaiC binds ATP and, with lower affinity, GTP. Point mutation by site- directed mutagenesis of P loop 1 completely nullified the circadian rhythm of kaiBC expression and markedly reduced ATP-binding activity. Moreover, KaiC can be autophosphorylated in vitro. These results suggest that the nucleotide-binding activity of KaiC plays important roles in the generation of circadian oscillation in cyanobacteria.

本文言語English
ページ(範囲)495-499
ページ数5
ジャーナルProceedings of the National Academy of Sciences of the United States of America
97
1
DOI
出版ステータスPublished - 2000 1月 4
外部発表はい

ASJC Scopus subject areas

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