Nucleotide kinase-based selection system for genetic switches

Kohei Ike, Daisuke Umeno

研究成果: Chapter

1 被引用数 (Scopus)

抄録

Ever-increasing repertories of RNA-based switching devices are enabling synthetic biologists to construct compact, self-standing, and easy-to-integrate regulatory circuits. However, it is rather rare that the existing RNA-based expression controllers happen to have the exact specification needed for particular applications from the beginning. Evolutionary design of is powerful strategy for quickly tuning functions/specification of genetic switches. Presented here are the steps required for rapid and efficient enrichment of genetic switches with desired specification using recently developed nucleoside kinase-based dual selection system. Here, the library of genetic switches, created by randomizing either the part or the entire sequence coding switching components, is subjected to OFF (negative) selection and ON (positive) selection in various conditions. The entire selection process is completed only by liquid handling, facilitating the parallel and continuous operations of multiple selection projects. This automation-liable platform for genetic selection of functional switches has potential applications for development of RNA-based biosensors, expression controllers, and their integrated forms (genetic circuits).

本文言語English
ホスト出版物のタイトルArtificial Riboswitches
ホスト出版物のサブタイトルMethods and Protocols
出版社Humana Press Inc.
ページ141-152
ページ数12
ISBN(印刷版)9781627037549
DOI
出版ステータスPublished - 2014
外部発表はい

出版物シリーズ

名前Methods in Molecular Biology
1111
ISSN(印刷版)1064-3745

ASJC Scopus subject areas

  • 分子生物学
  • 遺伝学

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