Nucleotide sequences of animal mitochondrial tRNAs(Met) possibly recognizing both AUG and AUA codons.

C. Takemoto*, T. Ueda, K. Miura, K. Watanabe

*この研究の対応する著者

研究成果: Article査読

15 被引用数 (Scopus)

抄録

To elucidate the role of modified nucleosides of tRNA in mitochondrial translation systems, especially with regard to their codon recognition, we purified mitochondrial tRNAs(Met) isolated from liver of frog, chicken and rat, and determined their nucleotide sequences. All of these tRNAs(Met) were found to possess 5-formylcytidine in the first letter of the anticodon, which is known to be prerequisite for bovine mt tRNA(Met) to decode AUA codon as well as AUG codon. These tRNA possesses two pseudeuridines in similar positions, and only chicken tRNA(Met) had ribothymidine at the first position of the T-loop, which is always found in the usual tRNAs. Considering that AUA codon is used as five times frequently as AUG codon in these animal mitochondrial genomes, it is deduced that 5-formylcytidine at the wobble position is essential for the recognition of both AUA and AUG codons.

本文言語English
ページ(範囲)77-78
ページ数2
ジャーナルNucleic acids symposium series
42
DOI
出版ステータスPublished - 1999
外部発表はい

ASJC Scopus subject areas

  • 医学(全般)

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