Occurrence of β-endorphin binding sites in the pituitary of the frog Rana esculenta: Effect of β-endorphin on luteinizing hormone secretion

M. G. Sabbieti, L. Marchetti, G. Menghi, K. Yamamoto, S. Kikuyama, H. Vaudry, A. Polzonetti-Magni*

*この研究の対応する著者

    研究成果: Article査読

    10 被引用数 (Scopus)

    抄録

    The possible effect of proopiomelanocortin-derived peptide, β-endorphin on frog gonadotrope cells was investigated. Binding and internalization of β-endorphin to pituitary pars distalis cultured cells were visualized by immunofluorescence and analyzed by means of confocal laser scanning microscopy. Using biotinylated endorphin, the time-course of β-binding showed that this opioid was internalized through receptor-mediated endocytosis, the mechanism in which actin and clathrin were involved; then, the lysosomal degradation program occurred at later stages. The β-endorphin binding was well antagonized by Naloxone, the opiate receptor antagonist, and up-regulated since more rapid response was obtained in the previously primed cells. The double immunostaining reaction for β-endorphin and LH β-subunit revealed that half the β-endorphin labeled cell population was positively immunostained for LH β-subunit, and β-endorphin was able to induce an increasing trend of LH secretion in cultured pars distalis cells. Therefore, it seems that β-endorphin acts directly on pituitary pars distalis and influences gonadotropin secretion through the interaction with its own receptor.

    本文言語English
    ページ(範囲)391-398
    ページ数8
    ジャーナルGeneral and Comparative Endocrinology
    132
    3
    DOI
    出版ステータスPublished - 2003 7月 1

    ASJC Scopus subject areas

    • 内分泌学

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