On-chip microcultivation chamber for swimming cells using visualized poly(dimethylsiloxane) valves

Kazunori Takahashi, Kazuki Orita, Kazunori Matsumura, Kenji Yasuda

研究成果: Letter

15 引用 (Scopus)

抄録

We have developed a new type of on-chip microcultivation chamber made of poly(dimethylsiloxane) (PDMS) for long-term cultivation of swimming cells. The advantages of this chamber are that (1) the microfluidic channel width of the valve can be controlled according to air pressure while monitoring the microscopic image of the channel width, and that (2) a simple single-step procedure is required for the fabrication of the valve structure and microfluidic pathway. Using this chamber, we can control the passage of swimming Chlamydomonas cells, through the channel while visualizing the opening and closing of the valve as the medium buffer passes any time. Thus the long-term observation of the behavior of a particular single cell is accomplished.

元の言語English
ジャーナルJapanese Journal of Applied Physics, Part 2: Letters
42
発行部数9 AB
出版物ステータスPublished - 2003 9 15
外部発表Yes

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Polydimethylsiloxane
Microfluidics
chambers
chips
cells
closing
Fabrication
Monitoring
buffers
Air
fabrication
air
Swimming

ASJC Scopus subject areas

  • Physics and Astronomy (miscellaneous)

これを引用

On-chip microcultivation chamber for swimming cells using visualized poly(dimethylsiloxane) valves. / Takahashi, Kazunori; Orita, Kazuki; Matsumura, Kazunori; Yasuda, Kenji.

:: Japanese Journal of Applied Physics, Part 2: Letters, 巻 42, 番号 9 AB, 15.09.2003.

研究成果: Letter

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abstract = "We have developed a new type of on-chip microcultivation chamber made of poly(dimethylsiloxane) (PDMS) for long-term cultivation of swimming cells. The advantages of this chamber are that (1) the microfluidic channel width of the valve can be controlled according to air pressure while monitoring the microscopic image of the channel width, and that (2) a simple single-step procedure is required for the fabrication of the valve structure and microfluidic pathway. Using this chamber, we can control the passage of swimming Chlamydomonas cells, through the channel while visualizing the opening and closing of the valve as the medium buffer passes any time. Thus the long-term observation of the behavior of a particular single cell is accomplished.",
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AU - Takahashi, Kazunori

AU - Orita, Kazuki

AU - Matsumura, Kazunori

AU - Yasuda, Kenji

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N2 - We have developed a new type of on-chip microcultivation chamber made of poly(dimethylsiloxane) (PDMS) for long-term cultivation of swimming cells. The advantages of this chamber are that (1) the microfluidic channel width of the valve can be controlled according to air pressure while monitoring the microscopic image of the channel width, and that (2) a simple single-step procedure is required for the fabrication of the valve structure and microfluidic pathway. Using this chamber, we can control the passage of swimming Chlamydomonas cells, through the channel while visualizing the opening and closing of the valve as the medium buffer passes any time. Thus the long-term observation of the behavior of a particular single cell is accomplished.

AB - We have developed a new type of on-chip microcultivation chamber made of poly(dimethylsiloxane) (PDMS) for long-term cultivation of swimming cells. The advantages of this chamber are that (1) the microfluidic channel width of the valve can be controlled according to air pressure while monitoring the microscopic image of the channel width, and that (2) a simple single-step procedure is required for the fabrication of the valve structure and microfluidic pathway. Using this chamber, we can control the passage of swimming Chlamydomonas cells, through the channel while visualizing the opening and closing of the valve as the medium buffer passes any time. Thus the long-term observation of the behavior of a particular single cell is accomplished.

KW - Air pressure

KW - Chlamydomonas

KW - Microcultivation chamber

KW - Microscopic monitoring

KW - Poly(dimethylsiloxane)

KW - Swimming cells

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