On-chip microcultivation chamber for swimming cells using visualized poly(dimethylsiloxane) valves

Kazunori Takahashi, Kazuki Orita, Kazunori Matsumura, Kenji Yasuda*

*この研究の対応する著者

研究成果: Letter査読

15 被引用数 (Scopus)

抄録

We have developed a new type of on-chip microcultivation chamber made of poly(dimethylsiloxane) (PDMS) for long-term cultivation of swimming cells. The advantages of this chamber are that (1) the microfluidic channel width of the valve can be controlled according to air pressure while monitoring the microscopic image of the channel width, and that (2) a simple single-step procedure is required for the fabrication of the valve structure and microfluidic pathway. Using this chamber, we can control the passage of swimming Chlamydomonas cells, through the channel while visualizing the opening and closing of the valve as the medium buffer passes any time. Thus the long-term observation of the behavior of a particular single cell is accomplished.

本文言語English
ページ(範囲)L1104-L1107
ジャーナルJapanese Journal of Applied Physics, Part 2: Letters
42
9 AB
出版ステータスPublished - 2003 9 15
外部発表はい

ASJC Scopus subject areas

  • 工学(全般)
  • 物理学および天文学(その他)
  • 物理学および天文学(全般)

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