On-chip neural cell cultivation using agarose-microchamber array constructed by a photothermal etching method

Hiroyuki Moriguchi*, Kazunori Takahashi, Yoshihiro Sugio, Yuichi Wakamoto, Ippei Inoue, Yasuhiko Jimbo, Kenji Yasuda

*この研究の対応する著者

研究成果: Article査読

27 被引用数 (Scopus)

抄録

We have developed a novel method for on-chip cultivation of neural cells in a flexible agarose-microchamber array on a glass slide. The agarose microchamber is a micrometer-order cavity constructed on the surface of an agarose layer by molding a 50-μm-high square/circular micro-cast of thick SU-8 photoresist. In addition, the shape of the agarose microchamber was rearranged by using the photothermal etching method, in which we used an infrared (1064-nm) focused laser beam as the heat source to melt and remove a portion of agarose gel at the heating spot. With the photothermal etching method, we can also manufacture narrow tunnel-shaped channels between microchambers. When nerve cells were cultured on the agar-microchamber array chip, the nerve cells in two adjacent microchambers connected through the photothermal-etched channel after 48 hours of cultivation. Those results suggest the potential of an agarose-microchamber array integrated with the photothermal etching method for the next stage of single cell cultivation and measurement of nerve cells, such as real-time control of cell interactions during cultivation.

本文言語English
ページ(範囲)37-42
ページ数6
ジャーナルElectrical Engineering in Japan (English translation of Denki Gakkai Ronbunshi)
146
2
DOI
出版ステータスPublished - 2004 1月 30
外部発表はい

ASJC Scopus subject areas

  • エネルギー工学および電力技術
  • 電子工学および電気工学

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