On-chip screening method for cell migration genes based on a transfection microarray

Reiko Onuki-Nagasaki, Akira Nagasaki, Kazumi Hakamada, Taro Q.P. Uyeda, Satoshi Fujita*, Masato Miyake, Jun Miyake

*この研究の対応する著者

研究成果: Article査読

24 被引用数 (Scopus)

抄録

Cell migration plays a major role in a variety of biological processes and a detailed understanding of associated mechanisms should lead to advances in the medical sciences, for example, in drug discovery for cancer therapy. However, the traditional methods used for analysis of cell migration cannot easily be scaled up for high-throughput screening. In this study, we have attempted to develop a novel simple method for high-throughput phenotypic screening for the identification of genes that are required for cell migration. As the appropriate cell line for the method, we found NBT-L2b cells that would be suitable for screening of migration-related genes in our method without influence by other cellular processes. Moreover, the idea for printing both the labeled fibronectin, for identification of the starting region of a cell, and the green fluorescent protein (GFP) expression vector, for identification of cells that had been transfected with siRNA and of the end point of migration, brings a rapid and efficient high-throughput screening procedure. Our new method will lead to an enhanced understanding of cell migration.

本文言語English
ページ(範囲)1502-1506
ページ数5
ジャーナルLab on a Chip
8
9
DOI
出版ステータスPublished - 2008
外部発表はい

ASJC Scopus subject areas

  • バイオエンジニアリング
  • 生化学
  • 化学 (全般)
  • 生体医工学

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