On-chip single-cell microcultivation assay for monitoring environmental effects on isolated cells

Senkei Umehara, Yuichi Wakamoto, Ippei Inoue, Kenji Yasuda*

*この研究の対応する著者

研究成果: Article査読

67 被引用数 (Scopus)

抄録

We have developed a on-chip single-cell microcultivation assay as a means of observing the adaptation process of single bacterial cells during nutrient concentration changes. This assay enables the direct observation of single cells captured in microchambers made on thin glass slides and having semipermeable membrane lids, in which cells were kept isolated with optical tweezers. After changing a medium of 0.2% (w/v) glucose concentration to make it nutrient-free 0.9% NaCl medium, the growth of all cells inserted into the medium stopped within 20min, irrespective of their cell cycles. When a nutrient-rich medium was restored, the cells started to grow again, even after the medium had remained nutrient-free for 42h. The results indicate that the cell's growth and division are directly related to their nutrient condition. The growth curve also indicates that the cells keep their memory of what their growth and division had been before they stopped growing.

本文言語English
ページ(範囲)534-540
ページ数7
ジャーナルBiochemical and Biophysical Research Communications
305
3
DOI
出版ステータスPublished - 2003 6 6
外部発表はい

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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