TY - JOUR
T1 - On-line monitoring of marine cyanobacterial cultivation based on phycocyanïn fluorescence
AU - Sode, Koji
AU - Horikoshi, Kenichi
AU - Takeyama, Haruko
AU - Nakamura, Noriyuki
AU - Matsunaga, Tadashi
PY - 1991/12
Y1 - 1991/12
N2 - A novel on-line fluorescence monitoring system for marine cyanobacterial cultivation was developed. This method is based on the measurement of intracellular phycocyanin content, which is the major light harvesting protein. A fluorescence spectrophotometer, equipped with a flow cell connected with a culture liquid recycling tube was used. Experiments were carried out using a marine unicellular cyanobacteria Synechococcus sp. NKBG 042902 isolated from Japanese coastal sea water. We have optimized excitation wavelength to avoid the light scattering, using non-pigmented old cells which no longer contained phycocyanin. At an excitation wavelength of 590 nm, light scattering was minimized. Viable cell concentration could be measured in the range of 2×106 to 2×108 cells per ml, without pronounced light scattering. Continuous monitoring of marine cyanobacteria cultivation was performed. Cell concentrations were determined by both culture fluorescence and by using a hemacytometer. A good linear correlation was obtained. We conclude that on-line monitoring of cyanobacterial culture fluorescence based on phycocyanin is a rapid, efficient and also versatile method for determining viable cell concentration.
AB - A novel on-line fluorescence monitoring system for marine cyanobacterial cultivation was developed. This method is based on the measurement of intracellular phycocyanin content, which is the major light harvesting protein. A fluorescence spectrophotometer, equipped with a flow cell connected with a culture liquid recycling tube was used. Experiments were carried out using a marine unicellular cyanobacteria Synechococcus sp. NKBG 042902 isolated from Japanese coastal sea water. We have optimized excitation wavelength to avoid the light scattering, using non-pigmented old cells which no longer contained phycocyanin. At an excitation wavelength of 590 nm, light scattering was minimized. Viable cell concentration could be measured in the range of 2×106 to 2×108 cells per ml, without pronounced light scattering. Continuous monitoring of marine cyanobacteria cultivation was performed. Cell concentrations were determined by both culture fluorescence and by using a hemacytometer. A good linear correlation was obtained. We conclude that on-line monitoring of cyanobacterial culture fluorescence based on phycocyanin is a rapid, efficient and also versatile method for determining viable cell concentration.
KW - Marine cyanobacteria
KW - On-line fluorescence monitoring
KW - Phycocyanin
KW - Synechococcus sp
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U2 - 10.1016/0168-1656(91)90042-T
DO - 10.1016/0168-1656(91)90042-T
M3 - Article
C2 - 1367694
AN - SCOPUS:0025935618
VL - 21
SP - 209
EP - 217
JO - Journal of Biotechnology
JF - Journal of Biotechnology
SN - 0168-1656
IS - 3
ER -