Optic nerve stimulation-induced increase of release of 3h-glutamate and 3h-aspartate but not 3h-gaba from the suprachiasmatic nucleus in slices of rat hypothalamus

Shyh Yuh Liou, Shigenobu Shibata, Katsunori Iwasaki, Showa Ueki

研究成果: Article

153 引用 (Scopus)

抄録

This study investigates the possibility of glutamate and/or aspartate as an excitatory transmitter in the retinohypothalamic fibers' terminal within the anterior hypothalamus, including the suprachiasmatic nucleus (SCN). We studied the release of [3]H-Glu and 3H-Asp from the SCN of rat hypothalamic slices produced by optic nerve stimulation. Stimulation of the optic nerve released 3H-Glu, 3H-Asp but not 3H-GABA, while stimulation of the SCN released all three. These releases were suppressed in both Ca2+ free Krebs and tetrodotoxin (TTX) 1 μM containing Krebs. The present result with previous electrophysiological observations strongly suggest that glutamate and/or aspartate are included in this retinohypothalamic termination, while GABA is included in intrinsic and/or extrinsic neurons of the SCN, excluding the terminal of the retinohypothalamic fibers.

元の言語English
ページ(範囲)527-531
ページ数5
ジャーナルBrain Research Bulletin
16
発行部数4
DOI
出版物ステータスPublished - 1986
外部発表Yes

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Suprachiasmatic Nucleus
Optic Nerve
Aspartic Acid
Hypothalamus
Glutamic Acid
gamma-Aminobutyric Acid
Anterior Hypothalamus
Tetrodotoxin
Neurons

ASJC Scopus subject areas

  • Neuroscience(all)

これを引用

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title = "Optic nerve stimulation-induced increase of release of 3h-glutamate and 3h-aspartate but not 3h-gaba from the suprachiasmatic nucleus in slices of rat hypothalamus",
abstract = "This study investigates the possibility of glutamate and/or aspartate as an excitatory transmitter in the retinohypothalamic fibers' terminal within the anterior hypothalamus, including the suprachiasmatic nucleus (SCN). We studied the release of [3]H-Glu and 3H-Asp from the SCN of rat hypothalamic slices produced by optic nerve stimulation. Stimulation of the optic nerve released 3H-Glu, 3H-Asp but not 3H-GABA, while stimulation of the SCN released all three. These releases were suppressed in both Ca2+ free Krebs and tetrodotoxin (TTX) 1 μM containing Krebs. The present result with previous electrophysiological observations strongly suggest that glutamate and/or aspartate are included in this retinohypothalamic termination, while GABA is included in intrinsic and/or extrinsic neurons of the SCN, excluding the terminal of the retinohypothalamic fibers.",
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author = "Liou, {Shyh Yuh} and Shigenobu Shibata and Katsunori Iwasaki and Showa Ueki",
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T1 - Optic nerve stimulation-induced increase of release of 3h-glutamate and 3h-aspartate but not 3h-gaba from the suprachiasmatic nucleus in slices of rat hypothalamus

AU - Liou, Shyh Yuh

AU - Shibata, Shigenobu

AU - Iwasaki, Katsunori

AU - Ueki, Showa

PY - 1986

Y1 - 1986

N2 - This study investigates the possibility of glutamate and/or aspartate as an excitatory transmitter in the retinohypothalamic fibers' terminal within the anterior hypothalamus, including the suprachiasmatic nucleus (SCN). We studied the release of [3]H-Glu and 3H-Asp from the SCN of rat hypothalamic slices produced by optic nerve stimulation. Stimulation of the optic nerve released 3H-Glu, 3H-Asp but not 3H-GABA, while stimulation of the SCN released all three. These releases were suppressed in both Ca2+ free Krebs and tetrodotoxin (TTX) 1 μM containing Krebs. The present result with previous electrophysiological observations strongly suggest that glutamate and/or aspartate are included in this retinohypothalamic termination, while GABA is included in intrinsic and/or extrinsic neurons of the SCN, excluding the terminal of the retinohypothalamic fibers.

AB - This study investigates the possibility of glutamate and/or aspartate as an excitatory transmitter in the retinohypothalamic fibers' terminal within the anterior hypothalamus, including the suprachiasmatic nucleus (SCN). We studied the release of [3]H-Glu and 3H-Asp from the SCN of rat hypothalamic slices produced by optic nerve stimulation. Stimulation of the optic nerve released 3H-Glu, 3H-Asp but not 3H-GABA, while stimulation of the SCN released all three. These releases were suppressed in both Ca2+ free Krebs and tetrodotoxin (TTX) 1 μM containing Krebs. The present result with previous electrophysiological observations strongly suggest that glutamate and/or aspartate are included in this retinohypothalamic termination, while GABA is included in intrinsic and/or extrinsic neurons of the SCN, excluding the terminal of the retinohypothalamic fibers.

KW - Electrophysiology

KW - Excitatory amino acids

KW - Release experiment

KW - Retinohypothalamic pathway

KW - Suprachiasmatic nucleus

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U2 - 10.1016/0361-9230(86)90182-6

DO - 10.1016/0361-9230(86)90182-6

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C2 - 2872946

AN - SCOPUS:0022550115

VL - 16

SP - 527

EP - 531

JO - Brain Research Bulletin

JF - Brain Research Bulletin

SN - 0361-9230

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