Organic Microbial Electrochemical Transistor Monitoring Extracellular Electron Transfer

Gábor Méhes*, Arghyamalya Roy, Xenofon Strakosas, Magnus Berggren, Eleni Stavrinidou, Daniel T. Simon

*この研究の対応する著者

研究成果: Article査読

24 被引用数 (Scopus)

抄録

Extracellular electron transfer (EET) denotes the process of microbial respiration with electron transfer to extracellular acceptors and has been exploited in a range of microbial electrochemical systems (MESs). To further understand EET and to optimize the performance of MESs, a better understanding of the dynamics at the microscale is needed. However, the real-time monitoring of EET at high spatiotemporal resolution would require sophisticated signal amplification. To amplify local EET signals, a miniaturized bioelectronic device, the so-called organic microbial electrochemical transistor (OMECT), is developed, which includes Shewanella oneidensis MR-1 integrated onto organic electrochemical transistors comprising poly(3,4-ethylenedioxythiophene):poly(styrenesulfonate) (PEDOT:PSS) combined with poly(vinyl alcohol) (PVA). Bacteria are attached to the gate of the transistor by a chronoamperometric method and the successful attachment is confirmed by fluorescence microscopy. Monitoring EET with the OMECT configuration is achieved due to the inherent amplification of the transistor, revealing fast time-responses to lactate. The limits of detection when using microfabricated gates as charge collectors are also investigated. The work is a first step toward understanding and monitoring EET in highly confined spaces via microfabricated organic electronic devices, and it can be of importance to study exoelectrogens in microenvironments, such as those of the human microbiome.

本文言語English
論文番号2000641
ジャーナルAdvanced Science
7
15
DOI
出版ステータスPublished - 2020 8月 1
外部発表はい

ASJC Scopus subject areas

  • 医学(その他)
  • 化学工学(全般)
  • 材料科学(全般)
  • 生化学、遺伝学、分子生物学(その他)
  • 工学(全般)
  • 物理学および天文学(全般)

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