Origin of individuality of two daughter cells during the division process examined by the simultaneous measurement of growth and swimming property using an on-chip single-cell cultivation system

Senkei Umehara, Ippei Inoue, Yuichi Wakamoto, Kenji Yasuda*

*この研究の対応する著者

研究成果: Article査読

20 被引用数 (Scopus)

抄録

We examined the origin of individuality of two daughter cells born from an isolated single Escherichia coli mother cell during its cell division process by monitoring the change in its swimming behavior and tumbling frequency using an on-chip single-cell cultivation system. By keeping the isolated condition of an observed single cell, we compared its growth and swimming property within a generation and over up to seven generations. It revealed that running speed decreased as cell length smoothly increased within each generation, whereas tumbling frequency fluctuated among generations. Also found was an extraordinary tumbling mode characterized by the prolonged duration of pausing in predivisional cells after cell constriction. The observed prolonged pausing may imply the coexistence of two distinct control systems in a predivisional cell, indicating that individuality of daughter cells emerges after a mother cell initiates constriction and before it gets physically separated into two new cell bodies.

本文言語English
ページ(範囲)1061-1067
ページ数7
ジャーナルBiophysical Journal
93
3
DOI
出版ステータスPublished - 2007 8月
外部発表はい

ASJC Scopus subject areas

  • 生物理学

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