Origin of magnetosome membrane: Proteomic analysis of magnetosome membrane and comparison with cytoplasmic membrane

Masayoshi Tanaka, Yoshiko Okamura, Atsushi Arakaki, Tsuyoshi Tanaka, Haruko Takeyama, Tadashi Matsunaga

研究成果: Article

106 引用 (Scopus)

抄録

Prokaryotes are known to have evolved one or more unique organelles. Although several hypotheses have been proposed concerning the biogenesis of these intracellular components, the majority of these proposals remains unclear. Magnetotactic bacteria synthesize intracellular magnetosomes that are enclosed by lipid bilayer membranes. From the identification and characterization of several surface and transmembrane magnetosome proteins, we have postulated that magnetosomes are derived from the cytoplasmic membrane (CM). To confirm this hypothesis, a comparative proteomic analysis of the magnetosome membrane (MM) and CM of the magnetotactic bacterium, Magnetospirillum magneticum AMB-1, was undertaken. Based on the whole genome sequence of M. magneticum AMB-1, 78 identified MM proteins were also found to be prevalent in the CM, several of which are related to magnetosome biosynthesis, such as Mms13, which is tightly bound on the magnetite surface. Fatty acid analysis was also conducted, and showed a striking similarity between the CM and MM profiles. These results suggest that the MM is derived from the CM.

元の言語English
ページ(範囲)5234-5247
ページ数14
ジャーナルProteomics
6
発行部数19
DOI
出版物ステータスPublished - 2006 10
外部発表Yes

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Magnetosomes
Proteomics
Cell Membrane
Membranes
Magnetospirillum
Bacteria
Ferrosoferric Oxide
Lipid bilayers
Biosynthesis
Lipid Bilayers
Membrane Lipids
Organelles
Membrane Proteins
Fatty Acids
Genes
Genome

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

これを引用

Origin of magnetosome membrane : Proteomic analysis of magnetosome membrane and comparison with cytoplasmic membrane. / Tanaka, Masayoshi; Okamura, Yoshiko; Arakaki, Atsushi; Tanaka, Tsuyoshi; Takeyama, Haruko; Matsunaga, Tadashi.

:: Proteomics, 巻 6, 番号 19, 10.2006, p. 5234-5247.

研究成果: Article

Tanaka, Masayoshi ; Okamura, Yoshiko ; Arakaki, Atsushi ; Tanaka, Tsuyoshi ; Takeyama, Haruko ; Matsunaga, Tadashi. / Origin of magnetosome membrane : Proteomic analysis of magnetosome membrane and comparison with cytoplasmic membrane. :: Proteomics. 2006 ; 巻 6, 番号 19. pp. 5234-5247.
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AU - Arakaki, Atsushi

AU - Tanaka, Tsuyoshi

AU - Takeyama, Haruko

AU - Matsunaga, Tadashi

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KW - Magnetospirillum magneticum AMB-1

KW - Magnetotactic bacteria

KW - Proteome analysis

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