Oxidative/heat stress enhanced production of chitosanase from Streptomyces griseus cells through its interaction with liposome

Kien Xuan Ngo, Hiroshi Umakoshi, Haruyuki Ishii, Huong Thi Bui, Toshinori Shimanouchi, Ryoichi Kuboi*

*この研究の対応する著者

研究成果: Article査読

3 被引用数 (Scopus)

抄録

The effective production and secretion of chitosanase from S. griseus cells, in the presence of hydrogen peroxide, were studied by the treatment of the cells with 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylcholine (POPC) liposomes under heat condition. The variation of the conformation and activity of the chitosanase caused by the interaction of liposomes with target chitosanase under stress condition was systematically investigated by using circular dichroism (CD) spectra and dielectric dispersion analysis (DDA). The effect of the oxidative stress (hydrogen peroxide) on the lipid and protein peroxide of cell membrane of S. griseus pretreated with and without liposomes under heat stress at 41 °C was further carried out. The possible utilization of membrane-membrane interaction between liposomes and cell membrane induced by the heat treatment was further investigated to enhance the production of chitosanase from S. griseus under oxidative stress condition.

本文言語English
ページ(範囲)471-476
ページ数6
ジャーナルJournal of Bioscience and Bioengineering
108
6
DOI
出版ステータスPublished - 2009 12
外部発表はい

ASJC Scopus subject areas

  • バイオテクノロジー
  • 応用微生物学とバイオテクノロジー
  • バイオエンジニアリング

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