Pak1 is involved in dendrite initiation as a downstream effector of Rac1 in cortical neurons

Kanehiro Hayashi, Toshio Ohshima*, Katsuhiko Mikoshiba

*この研究の対応する著者

研究成果: Article査読

57 被引用数 (Scopus)

抄録

Dendrite development in neurons is one of the bases for the formation of a complex neuronal network in the nervous system, and involvement of the Rho family GTPases, including Rac1, Cdc42, and RhoA, in dendrite formation has been demonstrated. One of the effectors of Rac1 and Cdc42, p21-activated kinase 1 (Pak1), is abundant in the brain; however, the function of Pak1 in neurons remains unknown. In order to clarify the roles of Pak1 in neurons, we introduced mutant Pak1 into immature neurons by a novel gene transfer technique using in utero electropora-tion. Introduction of constitutive active (CA)-Pak1 led to increase the number of dendrites, whereas introduction of dominant negative (DN)-Pak1 caused a reduction, and coexpression of CA-Rac1 and DN-Pak1 also resulted in a reduction in the number of dendrites. These results suggest that Pak1 regulates dendrite initiation as a downstream effector of Rac1.

本文言語English
ページ(範囲)579-594
ページ数16
ジャーナルMolecular and Cellular Neuroscience
20
4
DOI
出版ステータスPublished - 2002 1 1
外部発表はい

ASJC Scopus subject areas

  • 分子生物学
  • 細胞および分子神経科学
  • 細胞生物学

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