抄録
PAL31 is a nuclear protein expressed by various cell types. In the present study, the expression and function of PAL31 were examined in the cytokine-regulated growth of T and B cell lines. Treatment of the cells with mitogens [ovine PRL, recombinant rat placental lactogen-I (PL-I) and human IL-3] caused a dose-dependent increase in the expression of PAL31 mRNA in the PRL-dependent cell line Nb2, and IL-3 dependent cell line BaF3. A time-course study on synchronized Nb2 cells revealed that the expression of PAL31 is specific to the late G1 and S phases. Immunocytological studies revealed that PAL31 accumulates in the nuclei at the S phase. Furthermore, the antisense oligonucleotide for PAL31 severely inhibited the proliferation of Nb2 cells by inhibiting cells progressing to the S phase. Thus, PAL31 is a nuclear protein associated with cell cycle progression.
本文言語 | English |
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ページ(範囲) | 1048-1054 |
ページ数 | 7 |
ジャーナル | Biochemical and Biophysical Research Communications |
巻 | 280 |
号 | 4 |
DOI | |
出版ステータス | Published - 2001 |
外部発表 | はい |
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology