Parallel and passive distribution to arrayed microwells using self-regulating pinched flow

Kentaro Kawai, Masaki Kanai, Tatsuya Munaka, Hirohisa Abe, Akira Murakami, Shuichi Shoji

研究成果: Article査読

1 被引用数 (Scopus)

抄録

A parallel and passive (without any actuators or electrodes) cell distribution method for a cellular diagnostic microwell array was developed. This method can be used to simultaneously distribute cells evenly to arrayed microwells only by introducing cell suspensions into the inlet. To regulate the dispersion of the number of distributed cells into each microwell, we conceived a novel concept, the self-regulating pinched flow. The self-regulating pinched flow is realized in a functional microchannel, which consists of center micropillar colonnades and side channels. Computational fluid dynamics (CFD) simulations were carried out to optimize the design of the pinching area. We developed a prototype device that can be used to successfully distribute the beads uniformly in eight parallel microwells. The coefficient of variation (CV) of the distributed beads in the microwells was 29.3%.

本文言語English
ページ(範囲)281-288
ページ数8
ジャーナルSensors and Materials
20
6
出版ステータスPublished - 2008 12 1

ASJC Scopus subject areas

  • 器械工学
  • 材料科学(全般)

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