PDMS-CYTOP hybrid structure microwell array chip for total internal reflection fluorescence microscopy

A. Nakahara*, Y. Shirasaki, J. Mizuno, T. Sekiguchi, O. Ohara, S. Shoji

*この研究の対応する著者

研究成果: Conference contribution

1 被引用数 (Scopus)

抄録

A microwell arrry device with a hybrid structure of amorphour perfluoroplymer, CYTOP™, and polydimethylsiloxane (PDMS) which is specialized in total internal refllection fluorescence (TIRF) microscopy was developed. Since the CYTOP layer, whose refractive index is as same as that of water, is sandwiched between PDMS and glass substrate, leakage of incident light is avoided. PDMS layer upon the CYTOP enables high aspect ratio structures and easy integration of microfluidic components. Since direct bonding of CYTOP and PDMS is difficult to achieve, a chemical bonding method using aminosilane coupling reagent and 3-Glycoidoxypropyltriethoxysilane was applied. The bond strength was about 0.74 MPa. 24 × 50 microwell array was fabricated and their optical properties was evaluated. Measured TIRF microscopy results shows no interfere with incident light under the conventionally used conditions.

本文言語English
ホスト出版物のタイトル8th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2013
ページ743-746
ページ数4
DOI
出版ステータスPublished - 2013
外部発表はい
イベント8th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2013 - Suzhou, China
継続期間: 2013 4 72013 4 10

出版物シリーズ

名前8th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2013

Other

Other8th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2013
国/地域China
CitySuzhou
Period13/4/713/4/10

ASJC Scopus subject areas

  • 生化学、遺伝学、分子生物学(その他)
  • バイオテクノロジー

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